>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
75-85 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey uPAR Fc Chimera is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) binds with an ED50 of 15.0-90.0 ng/mL.
Recombinant Cynomolgus Monkey uPAR Fc Chimera Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey uPAR Fc Chimera Protein Binding Activity.
When Recombinant Cynomolgus Monkey UPAR Fc Chimera (Catalog # 10972-UK) is immobilized at 1.00 μg/mL (100 µL/well), Recombinant Human u-Plasminogen Activator/Urokinase (1310-SE) binds with an ED50 of 15.0-90.0 ng/mL.
Recombinant Cynomolgus Monkey uPAR Fc Chimera Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey uPAR Fc Chimera (Catalog # 10972-UK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-85 kDa and 150-170 kDa, respectively.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 1.00 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Urokinase plasminogen activator receptor (uPAR), also known as CD87, is a cysteine-rich cell surface glycoprotein belonging to the Ly6/uPAR (LU) superfamily and is involved in the activation of plasminogen to plasmin (1). Mature uPAR consists of an extracellular domain (ECD) with 3 LU domains (D1, D2, and D3) similar to Ly‑6 antigens and snake venom alpha -neurotoxins and a C-terminal glycosyl-phosphatidylinositol (GPI) anchor linked to the last residue of the third LU domain (1-3). The ECD of mature cynomolgus uPAR shares 95% amino acid sequence identity with human uPAR. Posttranslational modification generates several soluble uPAR fragments including uPAR, uPAR DIIDIII, and uPAR DI in human (1-3). The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin. The binding of uPAR with uPA initiates a proteolytic cascade resulting in the degradation of extracellular matrix components and stimulates tissue remodeling (4). The uPAR/uPA interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis (4). uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site (5, 6). uPAR expression is limited in normal human tissue but is highly expressed by diverse cancer cells and by non-malignant cells that infiltrate cancers and uPAR expression is associated with poor prognosis and increased risk of metastasis (7). uPAR is considered as a biomarker in many inflammatory diseases including cancer, cardiovascular diseases, chronic kidney diseases and diabetes (6).
Blasi, F. and Carmeliet, P. (2002) Nat Rev Mol. Cell Biol 3:932.
Leth, J.M. and Ploug, M. (2021) Front Cell Dev. Biol. 9:732015.
Behrendt, N. et al. (1996) J Biol. Chem. 271:22885.
Mahmood, N. et al. (2018) Front. Oncol. 8:24.
Smith, H. et al. (2010) Nat Rev Mol Cell Biol 11:23.
Desmedt, S. et al. (2017) Crit. Rev. Clin. Lab. Sci. 54:117.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics