Human embryonic kidney cell, HEK293-derived cynomolgus monkey R-Spondin 3 protein Met33-His273
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
34-44 kDa, under reducing conditions.
Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.4-10 ng/mL.
Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey R‑Spondin 3 Protein Binding Activity.
Recombinant Cynomolgus Monkey R-Spondin 3 (Catalog # 10948-RS) activates TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.4‑10 ng/mL.
Recombinant Cynomolgus Monkey R-Spondin 3 Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey R-Spondin 3 Protein (Catalog # 10948-RS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-44 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 250 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: R-Spondin 3
Roof plate-specific spondin 3 (RSPO3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin1), is member of the R-Spondin family of proteins which are involved in the activation and regulation of Wnt signaling pathway. The R-Spondin family, consisting of 4 members, are extracellular glycoproteins which share ~60% amino acid (aa) homology and a conserved protein structure (1). Similar to other R-Spondins, RSPO3 contains two adjacent cysteine-rich furin-like domains with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif and a C-terminal tail rich in basic residues. The furin-like domains are needed for beta -catenin stabilization (2, 3). Cynomologus RSPO3 shares 97% and 88% aa sequence identity with human and mouse RSPO3, respectively. While every member of the R-Spondin family positive modulators of Wnt/ beta -catenin signaling, each one has a distinct expression pattern (1 - 5). In mouse, RSPO3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (5, 7). R-Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (10). RSPO3 has also been identified as an oncogene (11).
Kim, K.-A. et al. (2006) Cell Cycle, 5:23.
Chen, J.-Z. et al. (2002) Mol. Biol. Rep. 29:287.
Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
Kazanskaya, O. et al. (2008) Development 135:3655.
Aoki, M. et al. (2007) Dev. Biol. 301:218.
Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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