Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Name: Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF
Brand: R&D Systems
SKU: 10948-RS

Catalog # 10948-RS | R&D Systems, Inc. a Bio-Techne Brand

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	XP_005551811.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Source	Human embryonic kidney cell, HEK293-derived cynomolgus monkey R-Spondin 3 protein Met33-His273
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met33
Predicted Molecular Mass	27 kDa
SDS-PAGE	27 kDa
Activity	Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.4-10 ng/mL.

Scientific Data Examples for Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

	Recombinant Cynomolgus Monkey R‑Spondin 3 Protein Binding Activity. Recombinant Cynomolgus Monkey R-Spondin 3 (Catalog # 10948-RS) activates TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.4‑10 ng/mL.
	Recombinant Cynomolgus Monkey R-Spondin 3 Protein SDS-PAGE. 2 μg/lane of Recombinant Cynomolgus Monkey R-Spondin 3 Protein (Catalog # 10948-RS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-44 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10948-RS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: R-Spondin 3

Roof plate-specific spondin 3 (RSPO3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin1), is member of the R-Spondin family of proteins which are involved in the activation and regulation of Wnt signaling pathway. The R-Spondin family, consisting of 4 members, are extracellular glycoproteins which share ~60% amino acid (aa) homology and a conserved protein structure (1). Similar to other R-Spondins, RSPO3 contains two adjacent cysteine-rich furin-like domains with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif and a C-terminal tail rich in basic residues. The furin-like domains are needed for beta -catenin stabilization (2, 3). Cynomologus RSPO3 shares 97% and 88% aa sequence identity with human and mouse RSPO3, respectively. While every member of the R-Spondin family positive modulators of Wnt/ beta -catenin signaling, each one has a distinct expression pattern (1 - 5). In mouse, RSPO3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (5, 7). R-Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (10). RSPO3 has also been identified as an oncogene (11).

Item	Value
References	Kim, K.-A. et al. (2006) Cell Cycle, 5:23. Chen, J.-Z. et al. (2002) Mol. Biol. Rep. 29:287. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306. Kazanskaya, O. et al. (2008) Development 135:3655. Aoki, M. et al. (2007) Dev. Biol. 301:218. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903. Theodorou, V. et al. (2007) Nat. Genet. 6:759.
Long Name	Roof Plate-specific Spondin 3
Entrez Gene IDs	84870 (Human); 72780 (Mouse); 102132009 (Cynomolgus Monkey)
Alternate Names	CRISTIN1, Cristin 1, FLJ14440, PWTSR, Protein with TSP type-1 repeat, R-Spondin 3, R-spondin 3 homolog (Xenopus laevis), R-spondin-3, RSPO3, RSpondin 3, Roof plate-specific spondin-3, THSD2, Thrombospondin type-1 domain-containing protein 2, hPWTSR, hRspo3, thrombospondin, type I, domain containing 2

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Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Catalog # 10948-RS | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Scientific Data Examples for Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: R-Spondin 3

Citations for Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Product Documents for Recombinant Cynomolgus Monkey R-Spondin 3 Protein, CF

Product Datasheets

COA

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