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Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF

Catalog # 10485-N2 | R&D Systems, Inc. a Bio-Techne Brand
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10485-N2-100

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Nectin-2/CD112 protein
Cynomolgus Monkey
(Gln32-Gly360)
Accession # XP_005589607.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln32 predicted

Predicted Molecular Mass

62 kDa

SDS-PAGE

68-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey Nectin‑2/CD112 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera  (Catalog # 9317-N2) binds with an ED50 of 0.1-0.6 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF

Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein Binding Activity

Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein Binding Activity

When Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Chimera (10485-N2) is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera (9276-DN) binds with an ED50 of 0.1-0.6 μg/mL.
Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein SDS-PAGE

Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Chimera Protein (Catalog # 10485-N2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-80 kDa and 130-160 kDa, respectively.

Formulation, Preparation and Storage

10485-N2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-2/CD112

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. Based on its similarity with human Nectin-2, cynomolgus Nectin-2/CD112 (Nectin-2 delta) is predicted to consist of a 329 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain (2). Within the ECD, cynomolgus Nectin-2 shares 96% aa sequence identity with human Nectin-2. Alternative splicing of human Nectin-2 generates a short 60 kDa isoform with a 94 aa cytoplasmic tail (2). Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts (1, 3). It forms homodimers in cis, followed by dimers in trans (between cells) (3). It does not cis-dimerize with other Nectins but forms cis-dimers between its two splice forms. Notably, a Nectin-2 cis-dimer on one cell can heterodimerize with a Nectin-3 cis-dimer on a neighboring cell (3). Nectin-2 additionally binds to DNAM-1/CD226 on NK cells and triggers NK cell cytolytic activity (4, 5). Nectin-2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus (3, 6). Nectin-2 is a component of cardiac intercalated discs and limits fibrosis and dysfunction resulting from pressure overload (7).

References

  1. Samanta, D. and S.C. Almo (2015) Cell. Mol. Life Sci. 72:645.
  2. Eberle, F. et al. (1995) Gene 159:267.
  3. Struyf, F. et al. (2002) J. Virol. 76:12940.
  4. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  5. Pende, D. et al. (2005) Mol. Immunol. 42:463.
  6. Warner, M.S. et al. (1998) Virology 246:179.
  7. Satomi-Kobayashi, S. et al. (2009) Hypertension 54:825.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Entrez Gene IDs

5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)

Gene Symbol

NECTIN2

UniProt

Product Documents for Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF

For research use only

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