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Recombinant Cynomolgus Monkey LAIR2 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10340-LR

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10340-LR-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey LAIR2 protein
Cynomolgus Monkey LAIR2
(Gln22-Ser152)
Accession # XP_005590391.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln22 deduced from Glu23 after deblocking.

Predicted Molecular Mass

41 kDa

SDS-PAGE

53-60 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Bovine Collagen I is coated at 10 µg/mL (100 μL/well), Recombinant Cynomolgus Monkey LAIR2 Fc Chimera (Catalog # 10340-LR) binds with an ED50 of 0.04-0.32 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey LAIR2 Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey LAIR2 Fc Chimera Protein Binding Activity

Recombinant Cynomolgus Monkey LAIR2 Fc Chimera Protein Binding Activity

When Bovine Collagen I is coated at 10 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey LAIR2 Fc Chimera (Catalog # 10340-LR) binds with an ED50 of 0.04-0.32 ng/mL.
Recombinant Cynomolgus Monkey LAIR2 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Monkey LAIR2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey LAIR2 Fc Chimera (Catalog # 10340-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-60 kDa and 100-120 kDa, respectively.

Formulation, Preparation and Storage

10340-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR2

LAIR2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. Cynomolgus LAIR-2 cDNA encodes a 152 amino acid (aa) polypeptide precursor. Based on the similarity with human LAIR2, it likely includes a 21 aa signal peptide and a 131 aa mature chain. Cynomolgus LAIR2 shares 78% aa identity with human LAIR2. When compared to LAIR1, its transmembrane counterpart, LAIR2 shares 83% aa identity across the signal sequence and extracellular domains (1-3). Although one is secreted and the other is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR2 may compete with LAIR1 for ligand binding (3, 4). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported. LAIR2 is a soluble collagen-receptor, and it can be detected in the synovial fluid of rheumatoid arthritis patients, urine of pregnant women, and as well as primary cells (5, 6). In vitro studies have demonstrated LAIR2 can compete with LAIR1 for the same collagen binding site and suggesting LAIR2 may play an important role in immune cell activation (5, 6). LAIR2 can interact with complement component 1q (C1q) and mannose-binding lectin (MBL) and act as a complement inhibitor for the treatment and prevention of antibody-mediated allograft rejection and antibody-mediated clinical conditions (7).

References

  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.
  3. Meyaard, L. et al. (1997) Immunity 7:283.
  4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.
  5. Lebbink, R.J. et al. (2008) J. Immunol. 180:1662.
  6. Olde Nordkamp, M.J. et al. (2011) Arthritis Rheum. 63:3749.
  7. Olde Nordkamp, M.J. et al. (2014) J. Innate Immun. 6:284.

Long Name

Leukocyte-associated Ig-like Receptor 2

Alternate Names

CD306

Entrez Gene IDs

3904 (Human); 102141809 (Cynomolgus Monkey)

Gene Symbol

LAIR2

UniProt

Additional LAIR2 Products

Product Documents for Recombinant Cynomolgus Monkey LAIR2 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey LAIR2 Fc Chimera Protein, CF

For research use only

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