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Recombinant Cynomolgus Monkey Klotho beta His Protein, CF

Catalog # 10428-KB | R&D Systems, Inc. a Bio-Techne Brand
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10428-KB-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived cynomolgus monkey Klotho beta protein
Phe53-Leu997, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe53

Predicted Molecular Mass

110 kDa

SDS-PAGE

110-130 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human FGF-21 (Catalog # 2539-FG) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Klotho beta His-tag (Catalog # 10428-KB) binds with an ED50 of 0.05-0.4 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey Klotho beta His Protein, CF

Recombinant Cynomolgus Monkey Klotho beta His Protein Binding Activity

Recombinant Cynomolgus Monkey Klotho beta His Protein Binding Activity

When Recombinant Human FGF-21 (Catalog # 2539-FG) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Klotho beta His-tag (Catalog # 10428-KB) binds with an ED50 of 0.05-0.4 µg/mL.
Recombinant Cynomolgus Monkey Klotho beta His Protein SDS-PAGE

Recombinant Cynomolgus Monkey Klotho beta His Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Klotho beta His-tag (Catalog # 10428-KB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa.

Formulation, Preparation and Storage

10428-KB
Formulation Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Background: Klotho beta

Klotho beta (KLB), a divergent structural member of the glycosidase I superfamily, is a type I transmembrane protein expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Klotho beta, along with the closely related Klotho, consists of a large extracellular domain (ECD) containing two glycosidase-like regions, a transmembrane domain, and a short intracellular tail. The two glycosidase-like regions of Klotho beta share high homology to the one beta-glycosidase family yet lack the critical active site Glu residues present in beta-glycosidases necessary for catalytic activity, though physiologically relevant enzymatic activity for Klotho beta has been suggested (2, 3). The ECD of mature cynomologus Klotho beta shares 97% and 80% amino acid sequence identity with the ECD of human and mouse Klotho beta, respectively. Similar to Klotho, Klotho beta helps to regulate multiple metabolic processes in mammals by acting as a co-receptor that facilitates binding between FGF19 subfamily members and their receptors (4). The Klotho beta mediated interaction of FGF19 (FGF15 in mouse) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7- alpha hydroxylase (Cyp7a1) and sterol 12- alpha hydroxylase (Cyp8b1) (5-7). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, up-regulating adipocyte insulin-dependent glucose uptake (5 - 8). Regulation of Klotho beta function has been suggested as a therapy for several cardiometabolic diseases (9).

References

  1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
  2. Ito, S. et al. (2000) Mech Dev. 98:115
  3. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
  4. Kuro-O, M. (2019) Nat Rev Nephrol. 15:27.
  5. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
  6. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  7. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
  8. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
  9. Min, X. et al. (2018) J. Biol. Chem 293:14678.

Alternate Names

KLB

Entrez Gene IDs

152831 (Human); 83379 (Mouse); 289625 (Rat); 102147155 (Cynomolgus Monkey)

Gene Symbol

KLB

UniProt

Additional Klotho beta Products

Product Documents for Recombinant Cynomolgus Monkey Klotho beta His Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey Klotho beta His Protein, CF

For research use only

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