Mouse myeloma cell line, NS0-derived cynomolgus monkey Klotho beta protein Phe53-Leu997, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
110-130 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA.
When Recombinant Human FGF-21 (Catalog # 2539-FG) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Klotho beta His-tag (Catalog # 10428-KB) binds with an ED50 of 0.05-0.4 μg/mL.
Recombinant Cynomolgus Monkey Klotho beta His Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey Klotho beta His Protein Binding Activity
When Recombinant Human FGF-21 (Catalog # 2539-FG) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Klotho beta His-tag (Catalog # 10428-KB) binds with an ED50 of 0.05-0.4 µg/mL.
Recombinant Cynomolgus Monkey Klotho beta His Protein SDS-PAGE
2 μg/lane of Recombinant Cynomolgus Monkey Klotho beta His-tag (Catalog # 10428-KB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
1 month, 2 to 8 °C under sterile conditions after opening.
Background: Klotho beta
Klotho beta (KLB), a divergent structural member of the glycosidase I superfamily, is a type I transmembrane protein expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Klotho beta, along with the closely related Klotho, consists of a large extracellular domain (ECD) containing two glycosidase-like regions, a transmembrane domain, and a short intracellular tail. The two glycosidase-like regions of Klotho beta share high homology to the one beta -glycosidase family yet lack the critical active site Glu residues present in beta -glycosidases necessary for catalytic activity, though physiologically relevant enzymatic activity for Klotho beta has been suggested (2, 3). The ECD of mature cynomologus Klotho beta shares 97% and 80% amino acid sequence identity with the ECD of human and mouse Klotho beta, respectively. Similar to Klotho, Klotho beta helps to regulate multiple metabolic processes in mammals by acting as a co-receptor that facilitates binding between FGF19 subfamily members and their receptors (4). The Klotho beta mediated interaction of FGF19 (FGF15 in mouse) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7- alpha hydroxylase (Cyp7a1) and sterol 12- alpha hydroxylase (Cyp8b1) (5-7). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, up-regulating adipocyte insulin-dependent glucose uptake (5 - 8). Regulation of Klotho beta function has been suggested as a therapy for several cardiometabolic diseases (9).
Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
Ito, S. et al. (2000) Mech Dev. 98:115
Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
Kuro-O, M. (2019) Nat Rev Nephrol. 15:27.
Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
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