Source	Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-17/IL-17A protein Gly24-Ala155
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly24
Predicted Molecular Mass	15 kDa
SDS-PAGE	15-25 kDa, under reducing conditions
Activity	Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 0.2-2 ng/mL

Recombinant Cynomolgus Monkey IL-17/IL-17A Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Monkey IL-17/IL-17A Protein Bioactivity Recombinant Cynomolgus Monkey IL‑17/IL‑17A (Catalog # 10164-IL) induces IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2-2 ng/mL.
	Recombinant Cynomolgus Monkey IL-17/IL-17A Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey IL-1/IL-17A (Catalog # 10164-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 15-25 kDa and 30-50 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10164-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature cynomolgus monkey IL-17A shares 96% amino acid sequence identity with human IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15). IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

Item	Value
References	Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479. Yao, Z. et al. (1995) J. Immunol. 155:5483. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593. Chang, S.H. and C. Dong (2007) Cell Res. 17:435. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447. Wright, J.F. et al. (2008) J. Immunol. 181:2799. Rong, Z. et al. (2009) Cell Res. 19:208. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271. Liang, S.C. et al. (2007) J. Immunol. 179:7791. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755. Hsu, H. et al. (2008) Nat. Immunol. 9:166. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857. Wang, L. et al. (2009) J. Exp. Med. 206:1457. Kryczek, I. et al. (2009) Blood 114:357.
Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names	CTLA-8, CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17, IL17A, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A

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Recombinant Cynomolgus Monkey IL-17/IL-17A Protein, CF

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