Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF

Name: Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF
Brand: R&D Systems
SKU: 10215-CL

Catalog # 10215-CL

Product Datasheet / COA / SDS

GS Linker, Defined heterodimer stoichiometry.

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Key Product Details

Source:	HEK293
Accession #:	NP_001274204 (IL-12p40) & XP_005546300 (IL-12p35)
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-12 protein

Cynomolgus Monkey IL-12p40 (Ile23-Ser328) Accession # NP_001274204	GGGSGGGSGGGS	Cynomolgus Monkey IL-12p35 (Arg57-Ser253) Accession # XP_005546300
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ile23

Predicted Molecular Mass

58 kDa

SDS-PAGE

65-78 kDa, under reducing conditions

Activity

Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 0.15-0.9 ng/mL.

Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF Scientific Data Examples

	Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) Bioactivity Recombinant Cynomolgus Monkey IL-12 (Catalog # 10215-CL) enhances IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 0.15-0.9 ng/mL.
	Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey IL-12 (10215-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-78 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10215-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit (1, 2). The cynomolgus monkey p35 subunit shares 93.4% sequence identity with the human homolog, and the p40 subunit shares 96.4% identity with its human homolog. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation (2, 3). Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent (1-3).

Item	Value
References	Hasegawa, H. et al. (2016) Front. Immunol. 7:479. Schurich, A. et al. (2017) Rheumatology. 57:246. Agarwal, P. (2009). J Immunol. 183:1695.
Long Name	Interleukin 12
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)
Alternate Names	CLMF p35, CLMF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12, IL-12 subunit p35, IL-12, subunit p35, IL12, IL12A, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, p35

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