Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey ErbB2/Her2 protein Thr23-Thr652, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
95-110 kDa, under reducing conditions
Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED50 for this effect is 0.7-7 μg/mL in the presence of 0.6 µg/mL Human ErbB2/Her2 Antibody (Trastuzumab, Catalog # MAB9589).
Recombinant Cynomolgus Monkey ErbB2/Her2 His-tag Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey ErbB2/Her2 His-tag Protein Bioactivity.
Recombinant Cynomolgus Monkey ErbB2/Her2 His-tag Protein (Catalog # 10613-ER) blocks anti-ErbB2 mediated inhibition of the SK-BR-3 human breast cancer cell line proliferation. The ED50 is 0.7-7 μg/mL in the presence of 0.6 μg/mL Trastuzumab (Human ErbB2/Her2 Antibody, MAB9589).
Recombinant Cynomolgus Monkey ErbB2/Her2 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Cynomolgus Monkey ErbB2/Her2 His-tag (Catalog # 10613-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-110 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
ErbB2, also called human epidermal growth factor receptor 2 (HER2) and Neu, is a type I membrane glycoprotein and is one of four members of the ErbB family of receptor tyrosine kinases. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Cynomolgus ErbB2 consists of an extracellular domain (ECD) with two ligand binding domains and two cystine rich domains, a transmembranee region and a cytoplasmic domain with a flexible juxtamembrane region followed by a protein kinase domain (1). The ECD of mature cynomolgus ErbB2 shares 99% amino acid sequence identity with the ECD of human ErbB2. ErbB2 signaling is associated with neurodegenerative diseases such as multiple sclerosis and Alzheimer disease when impaired, while over-expression of ErbB2 signaling is associated with development of cancers (2). ErbB2 is widely expressed in epithelial cells and has been primarily associated with breast carcinomas (3, 4). Over-expression of HER-2 also caused other cancers such as gastric (2,5), colorectal (6), ovary, lung, bladder, head and neck (7), and osteosarcoma (8). Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family to form higher affinity signaling complexes (9). ErbB2 can be shed from the cell surface by the protease ADAM10, resulting in a constitutively active truncated intracellular receptor (10). ERBB2 is used as biomarkers in several cancers and is an attractive oncogenic driver and therapeutic target in cancers (6).
Ferguson, K.M. (2008) Annu Rev Biophys. 37:353.
Boku, N. (2014) Gastric Cancer. 17:1.
Harari, D. and Yarden, Y. (2000) Oncogene 19:6102.
Bertucci, F. et al. (2004) Oncogene 23:2564
Palle J. et al. (2020) Drug 80(4):401.
De Cuyper, A. et al. (2020) Clin Colorectal Cancer. 19(2):65.
Vats, S. et al. (2018) Indian Journal Pathology & Microbiology.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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