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Recombinant Cynomolgus Monkey EGFR His-tag Protein, CF

Catalog # 10405-ER | R&D Systems, Inc. a Bio-Techne Brand
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10405-ER-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey EGFR protein
Leu25-Ser645, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

69 kDa

SDS-PAGE

95-109 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey EGFR His-tag (Catalog # 10405-ER) is immobilized at 20 μg/mL, 100 μL/well, the concentration of Recombinant Human Pro-EGF (aa 21-1023) Protein (Catalog # 4289-EG) that produces 50% of the optimal binding response is approximately 100-600 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey EGFR His-tag Protein, CF

Recombinant Cynomolgus Monkey EGFR His-tag Protein Binding Activity

Recombinant Cynomolgus Monkey EGFR His-tag Protein Binding Activity

When Recombinant Cynomolgus Monkey EGFR His-tag (Catalog # 10405-ER) is immobilized 20 µg/mL, 100 µL/well, the concentration of Recombinant Human Pro-EGF (aa21-1023) Protein (Catalog # 4289-EG) that produces 50% of the optimal binding response is approximately 100-600 ng/mL.
Recombinant Cynomolgus Monkey EGFR His-tag Protein SDS-PAGE

Recombinant Cynomolgus Monkey EGFR His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey EGFR His-tag (Catalog # 10405-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-109 kDa.

Formulation, Preparation and Storage

10405-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Epidermal growth factor receptor (EGFR) is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3, 4). The mature ECD of cynomolgus EGFR shares 99% and 90% amino acid sequence identity with human and mouse EGFR, respectively. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (5‑7). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (5-7). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (8).

References

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Guillaudeau, A. et al. (2012) PLoS One. 7:1.
  4. Reiter J.L. et al. (2001) Genomics 71:1.
  5. Freed, D. M. et al. (2017) Cell. 171:683.
  6. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  7. Faria, J.A. et al. (2016) BBRC. 478:39.
  8. Lee, C.K. et al. (2017) J. Thoracic Oncology. 12:403.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

Product Documents for Recombinant Cynomolgus Monkey EGFR His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey EGFR His-tag Protein, CF

For research use only

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