Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

Name: Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF
Brand: R&D Systems
SKU: 10027-AL
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10027-AL

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Source:	CHO
Accession #:	XP_005548303
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

Product Specifications for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey ALCAM/CD166 protein Trp28-Ala526, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Trp28
Predicted Molecular Mass	57 kDa
SDS-PAGE	67-89 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human Cynomolgus Monkey ALCAM/CD166 is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD6 Fc Chimera binds with an ED₅₀ of 10-50 ng/mL.

Scientific Data Examples for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

	Recombinant Cynomolgus Monkey ALCAM His-tag Protein Binding Activity When Recombinant Cynomolgus Monkey ALCAM (Catalog # 10027-AL) is coated onto a microplate at 1 µg/mL, Biotinylated Recombinant Human CD6 Fc Chimera binds with an ED₅₀ of 10-50 ng/mL.
	Recombinant Cynomolgus Monkey ALCAM His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey ALCAM/CD166 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-89 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10027-AL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ALCAM/CD166

ALCAM (activated leukocyte cell adhesion molecule), designated CD166, is a 100‑110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). The cynomologous ALCAM amino acid (aa) sequence includes a signal peptide, an extracellular domain (ECD) with two V‑type and three C2‑type Ig‑like domains, a transmembrane domain and a short cytoplasmic domain (1). Human ALCAM has several isoforms, including an isoform lacking most of the cytoplasmic domain and a secreted isoform (sALCAM) which antagonizes full‑length ALCAM (2, 3). Mature cynomologous ALCAM ECD shares 93% and 96% aa sequence identity with human and mouse/rat ALCAM, respectively. ALCAM is expressed on multiple cell types including thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte‑derived dendritic cells (1, 4). ALCAM mediates low‑affinity adhesion with itself or the cysteine‑rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1‑11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4⁺CD8⁺ T cells (6). Adhesion of ALCAM‑expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (4, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM expression on melanoma cell lines appears to be pro‑metastatic, but anti‑metastatic activity has been reported in breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM‑L1, both in the developing retina and invasive melanoma (2, 12).

Item	Value
References	Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213. van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671. Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315. Zimmerman, A.W. et al. (2006) Blood 107:3212. van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783. Castro, M.A.A. et al. (2007) J. Immunol. 178:4351. Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637. Cayrol, R. et al. (2008) Nat. Immunol. 9:137. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805. King, J.A. et al. (2010) Mol. Cancer 9:266. Buhusi, M. et al. (2009) J. Neurosci. 29:15630.
Long Name	Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs	214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names	ALCAM, CD166, CD166 antigen, MEMDMGC71733, activated leucocyte cell adhesion molecule, activated leukocyte cell adhesion moleculeFLJ38514

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Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

Scientific Data Examples for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ALCAM/CD166

Citations for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

Product Documents for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

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