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Recombinant Cynomolgus Monkey 5T4 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2280-TG

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2280-TG-100

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey 5T4 protein
Cynomolgus Monkey 5T4
(Thr35-Ser355)
Accession # Q4R8Y9
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr35

Predicted Molecular Mass

61 kDa

SDS-PAGE

86-98 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey 5T4 Fc Chimera is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human Frizzled-8 Fc Chimera binds with an ED50 of 4-24 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey 5T4 Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey 5T4 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Monkey 5T4 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey 5T4 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-98 kDa and 170-200 kDa, respectively.

Formulation, Preparation and Storage

2280-TG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5T4

5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling (1). Distinct regions on the 5T4 ectodomain which contains eight leucine-rich repeats are thought to antagonize Wnt signaling (2). 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis (3-5).  Cynomolgus 5T4 is a 420 amino acid (aa) protein that includes a 34 aa signal peptide, a 321 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD, cynomolgus 5T4 shares 95% and 78% aa sequence identity with human and mouse 5T4, respectively. It has been identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain (6). 5T4 inhibits Wnt/ beta-catenin signaling by binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles (1). Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1 (1).  In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8.

References

  1. Kagermeier-Schenk, B. et al. (2011) Dev. Cell. 21:1129.
  2. Zhao, Y. et al. (2014) Structure. 22:612.
  3. Starzynska, T. et al. (1994) Br. J. Cancer. 69:899.
  4. Naganuma, H. et al. (2002) Anticancer Res. 22:1033.
  5. Harris, J.L. et al. (2018) Int. J. Biochem. Cell Biol. 99:28.
  6. Awan, A. et al. (2002) BBRC 290:1030.

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)

Gene Symbol

TPBG

UniProt

Additional 5T4 Products

Product Documents for Recombinant Cynomolgus Monkey 5T4 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey 5T4 Fc Chimera Protein, CF

For research use only

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