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Recombinant Cynomolgus LILRB5/CD85c/LIR-8 Fc Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11102-T4

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11102-T4-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey LILRB5/CD85c/LIR-8 protein
Cynomolgus Monkey LILRB5/CD85c/LIR-8
(Gly24-Gly458)
Accession # XP_005590354.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

74 kDa

SDS-PAGE

86-99 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey LILRB5/CD85c/LIR-8 Fc Chimera (Catalog # 11102-T4) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Angiopoietin-like Protein 7/ANGPTL7  (Catalog # 914-AN) binds with an ED50 of 14.0-140 ng/mL.

Scientific Data Images for Recombinant Cynomolgus LILRB5/CD85c/LIR-8 Fc Protein, CF

Recombinant Cynomolgus Monkey LILRB5/CD85c/LIR-8 Fc Chimera Protein Binding Activity.

When Recombinant Cynomolgus Monkey LILRB5/CD85c/LIR-8 Fc Chimera Protein (Catalog # 11102-T4) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Angiopoietin‑like Protein 7/ANGPTL7 (914-AN) binds with an ED50 of 14.0‑140 ng/mL.

Recombinant Cynomolgus Monkey LILRB5/CD85c/LIR-8 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey LILRB5/CD85c/LIR-8 Fc Chimera Protein (Catalog # 11102-T4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-99 kDa and 170-200 kDa, respectively.

Formulation, Preparation and Storage

11102-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 week, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Background: LILRB5/CD85c/LIR-8

LIR-8, also known as CD85c and LILRB5, belongs to the Leukocyte immunoglobulin-like receptors (LILR) family of transmembrane glycoproteins involved in regulating immune responses (1,2). There are at least thirteen LILR family members and are divided into activating (LILRA) or inhibiting (LILRB) molecules (1,2). Based on its similarity with human LIR8, mature cynomolgus LIR8 is predicted to consist of an extracellular domain (ECD) with four Ig-like domains, a transmembrane segment, and a cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM).  The LILR family appears to be primate-specific receptors in terms of sequence homology. LIR8 is expressed on NK cells and in the tryptic granules of mast cells and negatively regulates immune cell activation (3, 4). It is present on the mast cell surface following cell activation and degranulation (4). Activated mast cells may also release soluble forms of LIR8 (3). LIR8 has also been shown to be expressed on T cells and induce CD8+ T cell proliferation (5).  Consistent with the demonstrated binding of LILRB2 to Angiopoietin-like 2 and 5 (6), R&D Systems in-house testing indicates that LIR8 binds to Angiopoietin-like 7. Recently, a common missense variant of LIR8 was found to be associated with statin intolerance and myalgia (7).

References

  1. Brown, D. et al. (2004) Tissue antigens. 64:215.
  2. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  3. Borges, L. et al. (1997) J. Immunol. 159:5192.
  4. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  5. Hogan, H. E. et al. (2016) Sci Rep. 6:21780.
  6. Zheng, J. et al. (2012) Nature 485:656.
  7. Siddigui, M. et al. (2017) Eur. Heart J. 38:3569.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Alternate Names

CD85c, LIR-8, LIR8

Entrez Gene IDs

10990 (Human); 102129427 (Cynomolgus Monkey)

Gene Symbol

LILRB5

UniProt

Additional LILRB5/CD85c/LIR-8 Products

Product Documents for Recombinant Cynomolgus LILRB5/CD85c/LIR-8 Fc Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus LILRB5/CD85c/LIR-8 Fc Protein, CF

For research use only

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