Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

Name: Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10226-LR
Rating: 4 (1 reviews)

Catalog # 10226-LR | R&D Systems, Inc. a Bio-Techne Brand

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Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	XP_015297320.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

Technical Data
Citations / Reviews (1)

Product Specifications for Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey LAIR1 protein

Cynomolgus Monkey LAIR1 (Gln22-His163) Accession # XP_015297320.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gln22, deduced from deblocked sequence at Glu23

Predicted Molecular Mass

42 kDa

SDS-PAGE

42 kDa

Activity

Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Cynomolgus Monkey LAIR1 Fc Chimera binds with an ED₅₀ of 1‑6 ng/mL.

Scientific Data Examples for Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

	Recombinant Cynomolgus LAIR1 Fc Chimera Protein Binding Activity When Bovine Collagen I is coated at 10 μg/mL, 100 μL/well, Recombinant Cynomolgus LAIR1 Fc Chimera (Catalog # 10226-LR) binds with an ED₅₀ of 1-6 ng/mL.
	Recombinant Cynomolgus LAIR1 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus LAIR1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-64 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10226-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). Cynomolgus LAIR-1 cDNA encodes a 296 amino acid (aa) polypeptide precursor. Based on the similarity with human LAIR1, it is likely to be a type I transmembrane (TM) protein containing a 21 aa signal sequence, a 144 aa extracellular domain (ECD), a 21 aa TM domain and a 110 aa cytoplasmic domain. Within ECD cynomolgus LAIR-1 shares 82% aa identity with human LAIR-1. LAIR-1 ECD includes one C2-type Ig-like domain. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). LAIR1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6).

Item	Value
References	Meyaard, L.(2003) J. Biol. Regul. Homeost. Agents 17:330. Meyaard, L. et al. (1997) Immunity 7:283. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828. Verbrugge, A. et al. (2003) Int. Immunol. 15:1349. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.
Long Name	Leukocyte-associated Ig-like Receptor 1
Entrez Gene IDs	3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)
Alternate Names	CD305, CD305 antigen, CD305leukocyte-associated Ig-like receptor 1, HLAIR1, LAIR-1, LAIR1, leukocyte-associated immunoglobulin-like receptor 1

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Product Documents for Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

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