Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF

Name: Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF
Brand: R&D Systems
SKU: 10426-AV

Catalog # 10426-AV

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	XP_005573729.1 (Integrin alpha V) & XP_005584667.1 (Int
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Integrin alpha V beta 3 protein

Cynomolgus Monkey Integrin alpha V (Phe31-Val992) Accession # XP_005573729.1	His-Pro	2x GGGSGGGS-Acidic Tail	6-His tag
Cynomolgus Monkey Integrin beta 3 (Gly27-Asp718) Accession # XP_005584667.1	His-Pro	2x GGGSGGGS-Basic tail	HA tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe31 (Integrin alpha V) & Gly27 (Integrin beta 3)

Predicted Molecular Mass

115 kDa (Integrin alpha V) & 86 kDa (Integrin beta 3)

SDS-PAGE

105-160 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human Vitronectin (Catalog # 2308-VN) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin alpha V beta 3 (Catalog # 10426-AV) binds with an ED₅₀ of 0.06-0.72 μg/mL.

Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein Binding Activity. When Recombinant Human Vitronectin (Catalog # 2308-VN) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin aV beta 3 (Catalog # 10426-AV) binds with an ED₅₀ of 0.06-0.72 µg/mL.
	Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein SDS-PAGE. 2 μg/lane of Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein (Catalog # 10426-AV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-160 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10426-AV

Formulation	Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V beta 3

Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (13). The noncovalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (24). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the Nterminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V betapropeller structure (4). The alpha V subunit contributes a "thigh and a calf" region, while the beta 3 subunit contains a PSI domain and four cysteinerich IEGF folds. The alpha V subunit domains termed thigh, calf1 and calf2 generate a "knee" region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by "inside out" signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Within the extracellular domain (ECD), cynomolgus alpha V shares 99% amino acid (aa) sequence identity with the 962 aa human alpha V, while cynomolgus beta 3 ECD shares almost 100% aa sequence identity with the 692 aa human beta 3 ECD. Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone, as well as promotes their apoptosis (8, 9). MCSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Cell entry of several viruses is mediated by alpha V beta 3 (4, 10). alpha V beta 3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF1 to promote cancer cell proliferation and invasiveness (11, 12).

Item	Value
References	Hynes, R. O. (2002) Cell 110:673. Serini, G. et al. (2006) Exp. Cell Res. 312:651. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88. Xiong, J. et al. (2001) Science 294:339. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415. Djaffar, I. et al. (1994) Biochem. J. 300:69. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433. Faccio, R. et al. (2003) J. Clin. Invest. 111:749. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533. Somanath, P.R. et al. (2009) Angiogenesis 12:177. Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106.
Entrez Gene IDs	3685 (Human)
Alternate Names	CD51, CD51 antigen, Integrin alpha V beta 3, MSK8, VNRADKFZp686A08142, Vitronectin receptor subunit alpha, antigen identified by monoclonal L230, integrin alpha-V, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

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