Recombinant Cynomolgus Integrin alpha 4 beta 1 Protein, CF

Name: Recombinant Cynomolgus Integrin alpha 4 beta 1 Protein, CF
Brand: R&D Systems
SKU: 10527-A4

Catalog # 10527-A4

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	XP_005573683.1 (Integrin alpha 4) & XP_005564991.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Integrin alpha 4 beta 1 Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Integrin alpha 4 beta 1 protein

Cynomolgus Monkey Integrin alpha 4 (Tyr34-Gln970) Accession #
XP_005573683.1

HP + 2x GGGSGGGS

Acidic Tail

6-His tag

Cynomolgus Monkey Integrin beta 1 (Gln21-Asp728) Accession # XP_005564991.1

HP + 2x GGGSGGGS

Basic Tail

HA-tag

N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Tyr34 (Integhrin alpha 4) & Gln21 inferred from enzymatic pyroglutamate treatment revealing Thr22 (Integrin beta 1)

Predicted Molecular Mass

113 kDa (Integrin alpha 4) & 88 kDa (Integrin beta 1)

SDS-PAGE

120-160 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human VCAM-1/CD106 Fc Chimera (Catalog # 862-VC) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin alpha 4 beta 1 (Catalog # 10527-A4) binds with an ED₅₀ of 4-40 ng/mL.

Recombinant Cynomolgus Integrin alpha 4 beta 1 Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Monkey Integrin alpha 4 beta 1 Protein Binding Activity. When Recombinant Human VCAM-1/CD106 Fc Chimera (862-VC) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin alpha 4 beta 1 (Catalog # 10527-A4) binds with an ED₅₀ of 4-40 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10527-A4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 4 beta 1

Integrin alpha 4 beta 1, also called VLA4, is an integrin family adhesion receptor that shares the beta 1 subunit with eleven other family members and the alpha 4 subunit with integrin alpha 4 beta 7 (1-4). The non-covalent heterodimer of 150 kDa alpha 4/CD49d and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits mediates cell adhesion to VCAM-1/CD106 on other cells and the CS-1 fragment of fibronectin in the extracellular matrix (2-4). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 4 beta -propeller to form a binding domain when the dimer is in active, extended and open conformation. Each subunit has a transmembrane sequence and a short cytoplasmic tail. Within the ECD, cynomolgus alpha 4 shares 97% sequence idently with human alpha 4, while cynomolgus beta 1 shares 99.9% sequence identity with human beta 1 ECD. Five alternate splice forms of the human beta 1 cytoplasmic domain, including one antagonistic form, vary by 12 to 48 aa and show differential expression patterns (5). Leukocytes (except for neutrophils), erythroid precursors and some non-hematopoietic cells such as epicardial, endothelial and smooth muscle precursors, Schwann cells, and chorionic cells express alpha 4 beta 1 (6-10). Deletion is lethal in the mouse embryo due to faulty placentation and development of the epicardium and coronary vessels (7, 10). In the adult, alpha 4 beta 1 primarily regulates immune cell migration (11-13). Circulating leukocyte alpha 4 beta 1 is rapidly activated by inflamed endothelial cells that present VCAM-1 and chemokines such as SDF-1 (11). This activation facilitates rolling, firm adhesion, and extravasation. Interfering with leukocyte migration via the therapeutic alpha 4 beta 1antibody Natalizumab can reduce the severity of autoimmune disorders such as multiple sclerosis (12). Natalizumab can also mobilize hematopoietic precursors from the bone marrow by impeding their interaction with stromal cell VCAM-1 (8, 12). During immune cell activation, alpha 4 beta 1 can function as a costimulatory molecule (13, 14).

Item	Value
References	Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619. Takada, Y. et al. (1989) EMBO J. 8:1361. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183. Takada, Y. et al. (2007) Genome Biol. 8:215. Svineng, G. et al. (1998) Biochem. J. 330:1255. Eshghi, S. et al. (2007) J. Cell Biol. 177:871. Yang, J.T. et al. (1995) Development 121:549. Sheppard, A.M. et al. (1994) Cell Adhes. Commun. 2:27. Haack, H. and R.O. Hynes (2001) Dev. Biol. 233:38. Grazioli, A. et al. (2006) Dev. Biol. 293:165. Alon, R. and K. Ley (2008) Curr. Opin. Cell Biol. 20:525. Bauer, M. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1920. Banerjee, E.R. et al. (2008) Exp. Hematol. 36:1004. Nguyen, K. et al. (2008) Immunity 28:810.
Entrez Gene IDs	3676 (Human)
Alternate Names	CD49d, IA4, Integrin alpha 4 beta 1

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