Does this enzyme have a tag?

Name: Recombinant Cynomolgus Fibroblast Activation Protein alpha
Brand: R&D Systems
SKU: 10278-SE

No, this enzyme does not have a tag. Please refer to the Source section on the product-specific page or product datasheet for sequence information.

Recombinant Cynomolgus Fibroblast Activation Protein alpha

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10278-SE

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Key Product Details

Source:	CHO
Accession #:	XP_005573377
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Cynomolgus Fibroblast Activation Protein alpha

Product Specifications for Recombinant Cynomolgus Fibroblast Activation Protein alpha

Source	Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Fibroblast Activation Protein alpha/FAP protein Leu26-Asp760
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu26
Predicted Molecular Mass	85 kDa
SDS-PAGE	87-94 kDa, under reducing conditions
Activity	Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2500 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Cynomolgus Fibroblast Activation Protein alpha

	Recombinant Cynomolgus Fibroblast Activation Protein alpha Enzyme Activity Recombinant Cynomolgus Fibroblast Activation Protein (Catalog # 10278-SE) is measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methlycoumarin (AMC).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10278-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that forms a homodimer and is structurally related to the dipeptidyl peptidase IV (DPPIV/CD26) family with a short cytoplasmic tail, a single transmembrane domain, and an extracellular domain that contains the active site (1-3). Within the extracellular domain, cynomolgous FAP shares 99.6% and 89.8% amino acid (aa) sequence identity with human and mouse FAP, respectively. It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (4, 5). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 4, 5) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (6). FAP is also known to interact with several surface molecules to play roles in cell signaling, cell invasion and migration (3). Although not detectible in normal tissues, FAP is elevated in activated stromal fibroblasts, tumor-associated macrophages, activated hepatic stellate cells and myofibroblasts during pathological conditions that include tissue remodeling such as most types of cancer, wound healing, arthritis, atherosclerosis, and fibrosis (1, 3, 4, 7-9). Targeting FAP with vaccines, antibodies, or pharmacologics impairs tumor progression in several cancer models making it a promising immunotherapy target (9-12).

Item	Value
References	Zi, F. et al. (2015) Mol. Med. Rep. 11:3203. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595. Lay, A.J. et. al. (2019) Front. Biosci. 24:1. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505. Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441. Keane, F.M. et al. (2011) FEBS J. 278:1316. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110. Bauer, S. et al. (2006) Arthritis Res. 8:R171. Jiang, G.M. et al. (2016) Oncotarget 7:33472. Cheng, J.D. et al. (2002) Cancer Res. 62:4767. Kraman, M. et al. (2010) Science 330:827. Busek, P. et al. (2018) Front. Biosci. 23:1933.
Entrez Gene IDs	2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)
Alternate Names	170 kDa melanoma membrane-bound gelatinase, DKFZp686G13158, DPPIV, EC 3.4.21.-, FAP, FAPA, Fibroblast Activation Protein alpha, Integral membrane serine protease, Seprase, fibroblast activation protein, alpha, vibronectin

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FAQs for Recombinant Cynomolgus Fibroblast Activation Protein alpha

Does this enzyme have a tag?

No, this enzyme does not have a tag. Please refer to the Source section on the product-specific page or product datasheet for sequence information.

Recombinant Cynomolgus Fibroblast Activation Protein alpha

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Cynomolgus Fibroblast Activation Protein alpha

Scientific Data Examples for Recombinant Cynomolgus Fibroblast Activation Protein alpha

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Fibroblast Activation Protein alpha/FAP

Citations for Recombinant Cynomolgus Fibroblast Activation Protein alpha

FAQs for Recombinant Cynomolgus Fibroblast Activation Protein alpha

Product Documents for Recombinant Cynomolgus Fibroblast Activation Protein alpha

Product Datasheets

COA

Certificate of Analysis

SDS

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