Does this enzyme have a tag?

Name: Recombinant Cynomolgus Fibroblast Activation Protein alpha
Brand: rnd
SKU: 10278-SE

No, this enzyme does not have a tag. Please refer to the Source section on the product-specific page or product datasheet for sequence information.

Recombinant Cynomolgus Fibroblast Activation Protein alpha

Catalog # 10278-SE

Datasheet / COA / SDS

Order Information

Bio-Techne Corporation

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Key Product Details

Source:	CHO
Accession #:	XP_005573377
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Cynomolgus Fibroblast Activation Protein alpha

Technical Data
Citations / Reviews / FAQs

Product Specifications

Source	Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Fibroblast Activation Protein alpha/FAP protein Leu26-Asp760
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu26
Predicted Molecular Mass	85 kDa
SDS-PAGE	87-94 kDa, under reducing conditions
Activity	Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2500 pmol/min/μg, as measured under the described conditions.

Recombinant Cynomolgus Fibroblast Activation Protein alpha Scientific Data Examples

	Recombinant Cynomolgus Fibroblast Activation Protein alpha Enzyme Activity Recombinant Cynomolgus Fibroblast Activation Protein (Catalog # 10278-SE) is measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methlycoumarin (AMC).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10278-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Assay Procedure

Materials

Assay Buffer: 50 mM Tris, 1 M NaCl, 1 mg/mL BSA, pH 7.5
Recombinant Cynomolgus Monkey Fibroblast Activation Protein alpha /FAP (cynoFAP) (Catalog # 10278-SE)
Substrate: Z-Gly-Pro-AMC (Bachem, Catalog # I-1145), 10 mM stock in DMSO
F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent

Dilute rcynoFAP to 0.2 µg/mL in Assay Buffer.
Dilute Substrate to 100 µM in Assay Buffer.
Load in plate 50 µL of 0.2 µg/mL rcynoFAP, and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 100 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode of 5 minutes.
Calculate specific activity:

Specific Activity (pmol/min/µg) =	Adjusted V_max* (RFU/min) x Conversion Factor** (pmol/RFU)
	amount of enzyme (µg)

*Adjusted for Substrate Blank
**Derived using calibration 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A9891).

Per Well:

rcynoFAP: 0.01 µg
Substrate: 50 µM

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that forms a homodimer and is structurally related to the dipeptidyl peptidase IV (DPPIV/CD26) family with a short cytoplasmic tail, a single transmembrane domain, and an extracellular domain that contains the active site (1-3). Within the extracellular domain, cynomolgous FAP shares 99.6% and 89.8% amino acid (aa) sequence identity with human and mouse FAP, respectively. It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (4, 5). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 4, 5) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (6). FAP is also known to interact with several surface molecules to play roles in cell signaling, cell invasion and migration (3). Although not detectible in normal tissues, FAP is elevated in activated stromal fibroblasts, tumor-associated macrophages, activated hepatic stellate cells and myofibroblasts during pathological conditions that include tissue remodeling such as most types of cancer, wound healing, arthritis, atherosclerosis, and fibrosis (1, 3, 4, 7-9). Targeting FAP with vaccines, antibodies, or pharmacologics impairs tumor progression in several cancer models making it a promising immunotherapy target (9-12).

Item	Value
References	Zi, F. et al. (2015) Mol. Med. Rep. 11:3203. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595. Lay, A.J. et. al. (2019) Front. Biosci. 24:1. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505. Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441. Keane, F.M. et al. (2011) FEBS J. 278:1316. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110. Bauer, S. et al. (2006) Arthritis Res. 8:R171. Jiang, G.M. et al. (2016) Oncotarget 7:33472. Cheng, J.D. et al. (2002) Cancer Res. 62:4767. Kraman, M. et al. (2010) Science 330:827. Busek, P. et al. (2018) Front. Biosci. 23:1933.
Entrez Gene IDs	2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)
Alternate Names	170 kDa melanoma membrane-bound gelatinase, DKFZp686G13158, DPPIV, EC 3.4.21.-, FAP, FAPA, Fibroblast Activation Protein alpha, Integral membrane serine protease, Seprase, fibroblast activation protein, alpha, vibronectin

Citations

Currently there are no citations for Recombinant Cynomolgus Fibroblast Activation Protein alpha.

FAQs

Does this enzyme have a tag?

No, this enzyme does not have a tag. Please refer to the Source section on the product-specific page or product datasheet for sequence information.