Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Name: Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF
Brand: R&D Systems
SKU: 10504-DC
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10504-DC

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Key Product Details

Source:	CHO
Accession #:	XP_015296329.1
Applications:	Bioactivity

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Product Specifications for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey DC-SIGN/CD209 protein

Hemagglutinin Tag (YPYDVPDYA)	Cynomolgus Monkey DC-SIGN/CD209 (Lys62-Glu406) Accession # XP_015296329.1
N-terminus	C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Tyr of HA tag

Predicted Molecular Mass

40 kDa

SDS-PAGE

44-54 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED₅₀ for this effect is 1.25-8 μg/mL.

Scientific Data Examples for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

	Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag Protein Bioactivity. Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (Catalog # 10504-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED₅₀ for this effect is 1.25-8 µg/mL.
	Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Cynomolgus Monkey DC-SIGN/CD209 HA-tag (10504-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-54 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10504-DC

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DC-SIGN/CD209

Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 40 kDa, 344 amino acid (aa) type II transmembrane protein, with the extracellular domain (ECD) containing a Ca2+-dependent carbohydrate-binding lectin domain (2). Within the ECD, cynomolgus DC-SIGN/CD209 shares 85% sequence identity with human DC-SIGN/CD209. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self-glycoproteins including ICAMs (2, 3). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (4,5).

Item	Value
References	Liu, W. et al. (2004) J. Biol. Chem. 279:18748. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571. Geijtenbeek, T.B. et al. (2000) Cell 100:575. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
Long Name	Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs	30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)
Alternate Names	CD209, CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin, CD209 molecule, CDSIGNHIV gpl20-binding protein, CLEC4L, CLEC4LC-type lectin domain family 4 member L, DC-SIGN, DC-SIGN1, DC-SIGN1C-type lectin domain family 4, member L, DC-SIGNMGC129965, DCSIGN, Dendritic cell-specific ICAM-3-grabbing non-integrin 1

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Product Documents for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

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Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Scientific Data Examples for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: DC-SIGN/CD209

Citations for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Customer Reviews for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF (1)

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Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

Product Documents for Recombinant Cynomolgus DC-SIGN/CD209 HA-tag Protein, CF

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