Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD96 protein Lys25-Met503, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
120-145 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD96 His-tag (Catalog # 10478-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) binds with an ED50 of 6-36 ng/mL.
Recombinant Cynomolgus CD96 His-tag Protein, CF Scientific Data Examples
Recombinant Cynomolgus CD96 His-tag Protein Binding Activity
When Recombinant Cynomolgus Monkey CD96 His-tag (10478-CD) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human CD155/PVR Fc Chimera (9174-CD) binds with an ED50 of 6-36 ng/mL.
Recombinant Cynomolgus CD96 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Cynomolgus CD96 His-tag Protein (10478-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-145 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
CD96, also known as Tactile (T cell-activated increased late expression), is a type I transmembrane glycoprotein belonging to the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). The extracellular domain (ECD) of CD96 contains three highly N- and O‑glycosylated Ig-like domains, with the two N-terminal domains being V-type and the distal domain a C-type structure (1). Within the mature ECD, cynomolgus CD96 shares 87% and 54% amino acid sequence identity with human and mouse CD96, respectively. In human, a splice variant with a 16 aa deletion in the second V-type domain, called CD96v2, can be expressed (2). CD96 is frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is also expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). On NK cells, co-stimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11).
Wang, P.L. et al. (1992) J. Immunol. 148:2600.
Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
Xie, W. et al. (2010) Cytometry A 77:840.
Eriksson, E. M. et al. (2012) PLOS One 7:e51696.
Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
Protein Accession # BAE32358.
Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
Fuchs, A. et al. (2004) J. Immunol. 172:3394.
Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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