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Recombinant Cynomolgus CD96 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10478-CD

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10478-CD-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD96 protein
Lys25-Met503, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys25

Predicted Molecular Mass

54 kDa

SDS-PAGE

120-145 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CD96 His-tag (Catalog # 10478-CD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Recombinant Human CD155/PVR Fc Chimera  (Catalog # 9174-CD) binds with an ED50 of 6-36 ng/mL.

Scientific Data Images for Recombinant Cynomolgus CD96 His-tag Protein, CF

Recombinant Cynomolgus CD96 His-tag Protein Binding Activity

Recombinant Cynomolgus CD96 His-tag Protein Binding Activity

When Recombinant Cynomolgus Monkey CD96 His-tag (10478-CD) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human CD155/PVR Fc Chimera (9174-CD) binds with an ED50 of 6-36 ng/mL.
Recombinant Cynomolgus CD96 His-tag Protein SDS-PAGE

Recombinant Cynomolgus CD96 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus CD96 His-tag Protein (10478-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-145 kDa.

Formulation, Preparation and Storage

10478-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD96

CD96, also known as Tactile (T cell-activated increased late expression), is a type I transmembrane glycoprotein belonging to the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). The extracellular domain (ECD) of CD96 contains three highly N- and O‑glycosylated Ig-like domains, with the two N-terminal domains being V-type and the distal domain a C-type structure (1). Within the mature ECD, cynomolgus CD96 shares 87% and 54% amino acid sequence identity with human and mouse CD96, respectively. In human, a splice variant with a 16 aa deletion in the second V-type domain, called CD96v2, can be expressed (2). CD96 is frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is also expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). On NK cells, co-stimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11).

References

  1. Wang, P.L. et al. (1992) J. Immunol. 148:2600.
  2. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  3. Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
  4. Xie, W. et al. (2010) Cytometry A 77:840.
  5. Eriksson, E. M. et al. (2012) PLOS One 7:e51696.
  6. Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
  7. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  8. Protein Accession # BAE32358.
  9. Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
  10. Fuchs, A. et al. (2004) J. Immunol. 172:3394.
  11. Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
  12. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

Additional CD96 Products

Product Documents for Recombinant Cynomolgus CD96 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus CD96 His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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