E. coli-derived cynomolgus monkey 4-1BB Ligand/TNFSF9 protein Arg68-Glu251, with an N-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
19-23 kDa, under reducing conditions
Recombinant Cynomolgus 4-1BB Ligand/TNFSF9 His Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey 4-1BB Ligand/TNFSF9 His-tag Protein Bioactivity.
When Recombinant Cynomolgus Monkey 4-1BB/TNFRSF9/CD137 Fc Chimera (9324-4B) is immobilized at 0.1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey 4-1BB Ligand/TNFSF9 His-tag (Catalog # 10439-4L) binds with an ED50 of 0.4-2.8 ng/mL.
Recombinant Cynomolgus Monkey 4-1BB Ligand/TNFSF9 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey 4-1BB Ligand/TNFSF9 His-tag Protein (Catalog # 10439-4L) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-23 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and TCEP with Trehalose.
Reconstitute at 500 μg/mL in water.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 4-1BB Ligand/TNFSF9
4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, cynomolgus Monkey 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10). 4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4+ and CD8+ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).
Wang, C. et al. (2009) Immunol. Rev. 229:192.
Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
Langstein, J. et al. (1998) J. Immunol. 160:2488.
Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
Baessler, T. et al. (2010) Blood 115:3058.
Gullo, C. et al. (2010) PLoS One 5:e10845.
Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
Salih, H.R. et al. (2001) J. Immunol. 167:4059.
Wen, T. et al. (2002) J. Immunol. 168:4897.
Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
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