Recombinant Canine IL-4 Protein

Name: Recombinant Canine IL-4 Protein
Brand: R&D Systems
SKU: 754-CL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 754-CL

Citations (20)

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Key Product Details

Source:	E. coli
Accession #:	AAD11563
Applications:	Bioactivity

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Product Specifications for Recombinant Canine IL-4 Protein

Source	E. coli-derived canine IL-4 protein His25-His132, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	12.9 kDa
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 3‑18 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

754-CL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

754-CL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1 - 3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Canine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature canine IL-4 shares 81%, 61%, 46%, 38%, and 37% aa sequence identity with feline, bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8-11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

Item	Value
References	Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1. Khatlani, T. et al. (1999) J. Vet. Med. Sci. 61:967. van der Kaaij, S.Y. et al. (1999) Immunogenetics 49:142. Redfield, C. et al. (1991) Biochemistry 30:11029. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701. Paludan, S.R. (1998) Scand. J. Immunol. 48:459. Corthay, A. (2006) Scand. J. Immunol. 64:93. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name	Interleukin 4
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names	B cell growth factor 1, B-cell stimulatory factor 1, BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL-4B_cell stimulatory factor 1, IL4, IL4E12, Lymphocyte stimulatory factor 1, MGC79402, binetrakin, interleukin 4, interleukin-4, pitrakinra

Citations for Recombinant Canine IL-4 Protein (20)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Canine IL-4 Protein include:

Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

M Pelst et al. (2022), Immortalised canine buccal epithelial cells' CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol Veterinary research, 53(1):72.
PMID: 36100942
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

CO Santos et al. (2021), Blocking IL-10 signaling with soluble IL-10 receptor restores in vitro specific lymphoproliferative response in dogs with leishmaniasis caused by Leishmania infantum PLoS ONE, 16(1):e0239171.
PMID: 33465107
Species: Canine
Sample Types: Whole Cells
Applications: Cell Culture

N Milhau et al. (2020), In vitro evaluations on canine monocyte-derived dendritic cells of a nanoparticles delivery system for vaccine antigen against Echinococcus granulosus PLoS ONE, 15(2):e0229121.
PMID: 32101539
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

LW Stranahan et al. (2020), Evaluation of the Efficacy of the Brucella canis RM6/66 deltavjbR Vaccine Candidate for Protection against B. canis Infection in Mice mSphere, 5(3).
PMID: 32434839
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

V Konduri et al. (2019), Dendritic cell vaccination plus low-dose doxorubicin for the treatment of spontaneous canine hemangiosarcoma Cancer Gene Ther., 0(0).
PMID: 30670791
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

M Pujol et al. (2019), Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation Comp. Immunol. Microbiol. Infect. Dis., 62(0):68-75.
PMID: 30711049
Species: Canine
Sample Types: Whole Blood
Applications: Control

EM van Helvoo et al. (2019), Canine IL4-10 fusion protein provides disease modifying activity in a canine model of OA; an exploratory study PLoS ONE, 14(7):e0219587.
PMID: 31295306
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

J Thalmensi et al. (2019), A DNA telomerase vaccine for canine cancer immunotherapy Oncotarget, 10(36):3361-3372.
PMID: 31164958
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

AJ Hernández- et al. (2018), Immunogenic potential of three transmissible venereal tumor cell lysates to prime canine-dendritic cells for cancer immunotherapy Res. Vet. Sci., 121(0):23-30.
PMID: 30316013
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

F Heinrich et al. (2017), Morphologic, phenotypic, and transcriptomic characterization of classically and alternatively activated canine blood-derived macrophages in vitro PLoS ONE, 12(8):e0183572.
PMID: 28817687
Species: Canine
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)

(2016), Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge PLoS ONE, 11(8):e0161169.
PMID: 27556586
Species: Canine
Sample Types: Recombinant Protein
Applications: ELISA (Standard)

(2016), Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs PLoS Negl Trop Dis, 10(5):e0004614.
PMID: 27223609
Species: Canine
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)

Costa-Pereira C et al. (2015), One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res, 11(0):92.
PMID: 25880646
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Qeska V et al. (2014), Canine distemper virus infection leads to an inhibitory phenotype of monocyte-derived dendritic cells in vitro with reduced expression of co-stimulatory molecules and increased interleukin-10 transcription. PLoS ONE, 9(4):e96121.
PMID: 24769532
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Xiong W et al. (2010), Human Flt3L generates dendritic cells from canine peripheral blood precursors: implications for a dog glioma clinical trial. PLoS ONE, 5(6):e11074.
PMID: 20552015
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Lange S et al. (2009), Investigation of immunological approaches to enhance engraftment in a 1 Gy TBI canine hematopoietic stem cell transplantation model. Exp. Hematol., 37(1):143-50.
PMID: 19100524
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Mason NJ et al. (2008), RNA-loaded CD40-activated B cells stimulate antigen-specific T-cell responses in dogs with spontaneous lymphoma. Gene Ther., 15(13):955-65.
PMID: 18337841
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Lemesre JL et al. (2007), Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial. Vaccine, 25(21):4223-34.
PMID: 17395339
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Lemesre JL et al. (2005), Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes. Vaccine, 23(22):2825-40.
PMID: 15780731
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Fujiwara RT et al. (2005), Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs. Vaccine, 24(4):501-9.
PMID: 16140437

Recombinant Canine IL-4 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Canine IL-4 Protein

Formulation, Preparation and Storage

Carrier Free

754-CL

Carrier

754-CL/CF

Carrier Free

Reconstitution Calculator

Background: IL-4

Citations for Recombinant Canine IL-4 Protein (20)

Product Documents for Recombinant Canine IL-4 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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