Recombinant A. tubingensis PNGase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9586-GH

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9586-GH-020
9586-GH-100
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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant A. tubingensis PNGase Protein (9586-GH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession #

OJI88926.1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all PNGase At Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived a. tubingensis PNGase At protein
Leu22-Ser557 (F78I, E352G, P381S, S406Q, T412S, S414F, S451T and S555T), with an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

60 kDa

SDS-PAGE

72-85 kDa, reducing conditions

Activity

Measured by its ability to deglycosylate ribonuclease B under denatured conditions.
50% ribonuclease B (6 μg) is deglycosylated by 500 ng of Recombinant A.tubingensis PNGase within 120 minutes, as measured under the described conditions.

Formulation, Preparation and Storage

9586-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: PNGase At

Glycoamidases are used extensively to deglycosylate asparagine-linked glycoproteins to obtain intact N-glycans and core proteins, thus are vital for scientists to understand the structure and function of these glycoproteins. A well known glycoamidase is peptide-N4-(N-acetyl-beta -D-glucosaminyl) asparaginase amidase F (PNGase F), an enzyme native to Flavobacterium meningosepticum (1). Another glycoamidase, native to fungus Aspergillus tubingensis, called PNGase At, has similar function but distinct substrate specificity coverage. Compared to PNGase F, PNGase At has broader substrate specificity, wider pH activity curve, and can deglycosylate those N-glycans with alpha1-3 fucosylated core structure, which is totally resistant to PNGase F (2).

References

  1. Elder, J.H. and Alexander, S. (1982) Proc. Natl. Acad. Sci. USA 79:4540.
  2. Ftouchi-Paquin, N. et al. (1997) J. Biol. Chem.  36:22960.

Long Name

Peptide-N4-(N-acetyl-beta-D-glucosaminyl asparagine amidase)

UniProt

OJI88926.1

Additional PNGase At Products

Product Documents for Recombinant A. tubingensis PNGase Protein, CF

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Product Specific Notices for Recombinant A. tubingensis PNGase Protein, CF

For research use only

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