I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

Name: Recombinant Human Nicotinic Acetylcholine R alpha 3/CHRNA3 His Protein
Brand: Novus Biologicals
SKU: NBP2-52109

A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Recombinant Human Nicotinic Acetylcholine R alpha 3/CHRNA3 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52109

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Nicotinic Acetylcholine R alpha 3/CHRNA3 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 32-240 of Human Nicotinic Acetylcholine R alpha 3/CHRNA3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSEAEHRL FERLFEDYNE IIRPVANVSD PVIIHFEVSM SQLVKVDEVN QIMETNLWLK QIWNDYKLKW NPSDYGGAEF MRVPAQKIWK PDIVLYNNAV GDFQVDDKTK ALLKYTGEVT WIPPAIFKSS CKIDVTYFPF DYQNCTMKFG SWSYDKAKID LVLIGSSMNL KDYWESGEWA IIKAPGYKHD IKYNCCEEIY PDITYSLYIR RL

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images

SDS-PAGE: Recombinant Human Nicotinic Acetylcholine R alpha 3/CHRNA3 His Protein [NBP2-52109]

SDS-Page: Nicotinic Acetylcholine R alpha 3/CHRNA3 Recombinant Protein [NBP2-52109] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-52109

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 3/CHRNA3

The nicotinic acetylcholine receptor (nAChR) is a ligand gated ion channel that mediates neurotransmission at the neuromuscular junction, autonomic ganglia and at some sites in the central nervous system. Distinct nAChR subtypes exist that can be stimulated by the neurotransmitter acetylcholine, the natural product nicotine, or by synthetic compounds. After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 3 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane.

Long Name

Nicotinic Acetylcholine Receptor alpha 3

Alternate Names

CHRNA3, LNCR2, NACHRA3, Nicotinic Acetylcholine Ra3, PAOD2

Gene Symbol

CHRNA3

Additional Nicotinic Acetylcholine R alpha 3/CHRNA3 Products

Product Documents

Product Datasheets

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COA

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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