Recombinant Human MLF1 Interacting Protein His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23247

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NBP2-23247
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MLF1 Interacting Protein Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 147-418 of Human MLF1 Interacting Protein

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MLF1 Interacting Protein His Protein

SDS-PAGE: Recombinant Human MLF1 Interacting Protein His Protein [NBP2-23247]

SDS-PAGE: Recombinant Human MLF1 Interacting Protein His Protein [NBP2-23247]

SDS-Page: Recombinant Human MLF1 Interacting Protein His Protein [NBP2-23247] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-23247
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLF1 Interacting Protein

MLF1IP, as known as centromere, is a specialized chromatin domain, present throughout the cell cycle that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. It plays an important role in the correct PLK1 localization to the mitotic kinetochores. It is a scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. It also involved in transcriptional repression. Recombinant human MLF1IP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

CENP-50, CENP-U, CENPU50, CENPUMLF1-interacting protein, centromere protein of 50 kDa, centromere protein U, FLJ23468, ICEN24, Interphase centromere complex protein 24, KLIP1CENP50, KSHV latent nuclear antigen interacting protein 1, KSHV latent nuclear antigen-interacting protein 1, MLF1 interacting protein, PBIP1, Polo-box-interacting protein 1

Gene Symbol

CENPU

Additional MLF1 Interacting Protein Products

Product Documents for Recombinant Human MLF1 Interacting Protein His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MLF1 Interacting Protein His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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