Recombinant Human MDH2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48325

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NBP1-48325-0.05mg
NBP1-48325-0.250mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all MDH2 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-338 of Human MDH2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

35.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 700 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MDH2 His Protein

SDS-PAGE: Recombinant Human MDH2 His Protein [NBP1-48325]

SDS-PAGE: Recombinant Human MDH2 His Protein [NBP1-48325]

SDS-Page: Recombinant Human MDH2 Protein [NBP1-48325] - 15% SDS-PAGE (3 ug).

Formulation, Preparation and Storage

NBP1-48325
Formulation 20 mM Tris-HCl buffer (pH 7.5), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDH2

MDH2 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant human MDH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Malate dehydrogenase, mitochondrial

Alternate Names

EC 1.1.1.37

Gene Symbol

MDH2

Additional MDH2 Products

Product Documents for Recombinant Human MDH2 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MDH2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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