Recombinant Mouse Legumain/Asparaginyl Endopeptidase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52295

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NBP2-52295-0.25mg
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Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Legumain/Asparaginyl Endopeptidase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 18-435 of Mouse Legumain/Asparaginyl Endopeptidase

Source: Baculovirus

Amino Acid Sequence: VPVGVDDPED GGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEENPTPGV VINRPNGTDV YKGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLKS GPRDHVFIYF TDHGATGILV FPNDDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMM NHLPDDINVY ATTAANPKES SYACYYDEER GTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHRASSPIS LPPVTHLDLT PSPDVPLTIL KRKLLRTNDV KESQNLIGQI QQFLDARHVI EKSVHKIVSL LAGFGETAER HLSERTMLTA HDCYQEAVTH FRTHCFNWHS VTYEHALRYL YVLANLCEAP YPIDRIEMAM DKVCLSHYLE HHHHHH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

48.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Legumain/Asparaginyl Endopeptidase His Protein

SDS-PAGE: Recombinant Mouse Legumain/Asparaginyl Endopeptidase His Protein [NBP2-52295]

SDS-PAGE: Recombinant Mouse Legumain/Asparaginyl Endopeptidase His Protein [NBP2-52295]

SDS-Page: Recombinant Mouse Legumain/Asparaginyl Endopeptidase Protein [NBP2-52295]

Formulation, Preparation and Storage

NBP2-52295
Formulation PBS (pH 7.4), 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Legumain/Asparaginyl Endopeptidase

Legumain encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq]

Alternate Names

AEP, Asparaginyl Endopeptidase, LGMN

Gene Symbol

LGMN

Additional Legumain/Asparaginyl Endopeptidase Products

Product Documents for Recombinant Mouse Legumain/Asparaginyl Endopeptidase His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Legumain/Asparaginyl Endopeptidase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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