Human Plasminogen Protein, CF

Name: Human Plasminogen Protein, CF
Brand: R&D Systems
SKU: 1939-SE
Price: 186 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1939-SE

Citations (4)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	N/A
Applications:	Enzyme Activity

View Full Technical Details for Human Plasminogen Protein, CF

Product Specifications for Human Plasminogen Protein, CF

Source	Plasminogen protein The human plasma used for the isolation of this product was certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	EPLDDYNVTQ (major) and LDDYNVTQGA (minor)
SDS-PAGE	100-106 kDa doublet, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, SUC-AFK-AMC. The specific activity, measured under the described conditions, is >500 pmol/min/µg.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1939-SE

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 1 mg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 8.0.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Plasminogen

Plasminogen (PLG) is the precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion (1, 2). Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. Activated by u- or t-plasminogen activator, the single-chain PLG (amino acid residues 20‑810) is converted to plasmin, which consists of disulfide bond-linked heavy chain A (residues 20-580) and light chain B (residues 581‑810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named as angiostatin, a novel angiogenesis inhibitor (3, 4).

Item	Value
References	Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104. Forsgren, M. et al. (1987) FEBS Lett. 213:254. O’Reilly, M.S. et al. (1994) Cell 79:315. Sim, B.K. et al. (1997) Cancer Res. 57:1329.
Entrez Gene IDs	5340 (Human); 18815 (Mouse); 85253 (Rat)
Alternate Names	DKFZp779M0222, EC 3.4.21, EC 3.4.21.7, Plasminogen, Plg, plasmin

Citations for Human Plasminogen Protein, CF (4)

Showing 1 - 4 of 4 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human Plasminogen Protein, CF include:

Species: Human
Sample Types: Plasma
Applications: ELISA Standard

XC Lim et al. (2022-01-01), Temporal Changes in Extracellular Vesicle Hemostatic Protein Composition Predict Favourable Left Ventricular Remodeling after Acute Myocardial Infarction International Journal of Molecular Sciences, 24(1).
PMID: 36613770
Species: Complex Species Category
Sample Types: Whole Cells
Applications: Bioassay

S El Chamy M et al. (2020-01-01), Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes Malar. J., 19(1):302.
PMID: 32847585
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

N Pamir et al. (2017-01-01), Plasminogen promotes cholesterol efflux by the ABCA1 pathway JCI Insight, 2(15).
PMID: 28768900
Species: Bacteria - Francisella tularensis
Sample Types: Buffer
Applications: ELISA Developmet

Crane DD et al. (2009-01-01), A novel role for plasmin-mediated degradation of opsonizing antibody in the evasion of host immunity by virulent, but not attenuated, Francisella tularensis. J. Immunol., 183(7):4593-600.
PMID: 19752236

There are no citations that match your criteria.

Showing 1 - 4 of 4 citations Showing All

Product Documents for Human Plasminogen Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS