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Key Product Details

Species Reactivity

Rat

Applications

Control

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 54447

Product Summary for Rat IgG2A Fluorescein-conjugated Antibody

Immunogen

KLH

Specificity

Selected for use as rat IgG2A isotype control for flow cytometry applications.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Rat IgG2A Fluorescein-conjugated Antibody

Detection of Rat IgG Isotype Control by Flow Cytometry

Detection of Rat IgG Control by Flow Cytometry

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB17582F, filled histogram) or Rat IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat IgG2A Fluorescein-conjugated Antibody

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using IC006F in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Rat IgG2A Fluorescein-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat IgG2A Fluorescein-conjugated Antibody

For research use only

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