trans-Ned 19
Tocris Bioscience, part of Bio-Techne | Catalog # 3954
NAADP antagonist; inhibits Ca2+ release
Key Product Details
Description
NAADP antagonist; inhibits Ca2+ release
Product Description
trans-Ned 19 is a nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist. Inhibits NAADP-mediated Ca2+ release (IC50 = 6 nM); also inhibits [32P]NAADP binding (IC50 = 0.4 nM). Fluorescently labels NAADP receptors in intact cells. Stereosiomer of cis-Ned 19 (Cat. No. 3953).Optical Data
| λabs | 368 nm |
| λem | 425 nm |
| Closest Laser line | 355 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy |
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Product Specifications
Molecular Weight
514.59
Formula
C30H31FN4O3
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(1R,3S)-1-[3-[[4-(2-Fluorophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
CAS Number
1354235-96-3
PubChem ID
1427628
InChI Key
FUHCEERDBRGPQZ-LBNVMWSVSA-N
SMILES
FC1=CC=CC=C1N(CC6)CCN6CC2=C[C@@]([C@@H]3C4=C(C(C=CC=C5)=C5N4)C[C@@H](C(O)=O)N3)=CC=C2OC
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 51.46 | 100 |
Preparing Stock Solutions
for trans-Ned 19
The following data is based on the product molecular weight 514.59
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.94 mL | 9.72 mL | 19.43 mL |
| 5 mM | 0.39 mL | 1.94 mL | 3.89 mL |
| 10 mM | 0.19 mL | 0.97 mL | 1.94 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.39 mL |
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Background References
References are publications that support the biological activity of the product.
- Rosen Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist Ned-19 indicate two binding sites on the NAADP receptor. J.Biol.Chem. 2009 PMID: 19826006
- Thai NAADP receptors mediate calcium signaling stimulated by endothelin-1 and NE in renal afferent arterioles. Am.J.Physiol.Renal Physiol. 2009 PMID: 19439521
- Naylor Identification of a chemical probe for NAADP by virtual screening. Nat.Chem.Biol. 2009 PMID: 19234453
Product Specific Notices for trans-Ned 19
For research use only
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