cis-Ned 19

Name: cis -Ned 19
Brand: Tocris Bioscience
SKU: 3953

Tocris Bioscience, part of Bio-Techne | Catalog # 3953

Citations (3)

COA / SDS

NAADP antagonist; inhibits Ca2+ release

Discontinued Product

3953 has been discontinued. View all Calcium Signaling Agents products.

Key Product Details

Description:	NAADP antagonist; inhibits Ca²⁺ release
Chemical Name:	(1S,3S)-1-[3-[[4-(2-Fluorophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
Purity:	≥97% (HPLC)
Molecular Weight:	514.59

View Full Technical Details for cis-Ned 19

Technical Data
Citations

Biological Activity for cis-Ned 19

cis-Ned 19 is an irreversible nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist; inhibits Ca²⁺ release (IC₅₀ = 800 nM) and [³²P]NAADP binding (IC₅₀ = 15 μM). Stereoisomer of trans-Ned 19. Fluorescently labels NAADP receptors in intact cells (excitation 351 and 365 nm using an ultraviolet Argon laser).

Isomer of trans-Ned 19.

Optical Data for cis-Ned 19

λ_abs	368 nm
λ_em	425 nm
Closest Laser line	355 nm
Cell Permeable	Yes
Application	Confocal Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for cis-Ned 19

M. Wt	514.59
Formula	C₃₀H₃₁FN₄O₃
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	1137264-00-6
PubChem ID	1427626
InChi Key	FUHCEERDBRGPQZ-LSYYVWMOSA-N
Smiles	FC1=CC=CC=C1N(CC6)CCN6CC2=C[C@@]([C@H]3C4=C(C(C=CC=C5)=C5N4)C[C@@H]([C@@](O)=O)N3)=CC=C2OC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for cis-Ned 19

References are publications that support the biological activity of the product.

Naylor Identification of a chemical probe for NAADP by virtual screening. Nat.Chem.Biol. 2009 PMID: 19234453