cis-Ned 19

Name: cis -Ned 19
Brand: Tocris Bioscience
SKU: 3953

Tocris Bioscience a Bio-Techne Brand

Catalog # 3953

Citations (2)

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Key Product Details

Description:	Fluorescently labels NAADP receptors in intact cells; inhibits Ca²⁺ release
Chemical Name:	(1S,3S)-1-[3-[[4-(2-Fluorophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
Purity:	≥97% (HPLC)
Molecular Weight:	514.59

View Full Technical Details for cis-Ned 19

Biological Activity for cis-Ned 19

cis-Ned 19 is an irreversible nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist; inhibits Ca²⁺ release (IC₅₀ = 800 nM) and [³²P]NAADP binding (IC₅₀ = 15 μM). Stereoisomer of trans-Ned 19. Fluorescently labels NAADP receptors in intact cells (excitation 351 and 365 nm using an ultraviolet Argon laser).

Isomer of trans-Ned 19.

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Technical Data for cis-Ned 19

M. Wt	514.59
Formula	C₃₀H₃₁FN₄O₃
Storage	Store at -20°C
Purity	≥97% (HPLC)
CAS Number	1137264-00-6
PubChem ID	1427626
InChi Key	FUHCEERDBRGPQZ-LSYYVWMOSA-N
Smiles	FC1=CC=CC=C1N(CC6)CCN6CC2=C[C@@]([C@H]3C4=C(C(C=CC=C5)=C5N4)C[C@@H]([C@@](O)=O)N3)=CC=C2OC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for cis-Ned 19

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	51.46	100

Preparing Stock Solutions for cis-Ned 19

The following data is based on the product molecular weight 514.59.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.94 mL	9.72 mL	19.43 mL
5 mM	0.39 mL	1.94 mL	3.89 mL
10 mM	0.19 mL	0.97 mL	1.94 mL
50 mM	0.04 mL	0.19 mL	0.39 mL

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References for cis-Ned 19

References are publications that support the biological activity of the product.

Naylor Identification of a chemical probe for NAADP by virtual screening. Nat.Chem.Biol. 2009 PMID: 19234453

Citations for cis-Ned 19 (2)

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Citations are publications that use Bio-Techne products. Selected citations for cis-Ned 19 include:

Gerasimenko et al. (2015) Both RyRs and TPCs are required for NAADP-induced intracellular Ca2+ release. Sci Rep. 58:237
PMID: 26100948
Favia et al. (2014) VEGF-induced neoangiogenesis is mediated by NAADP and two-pore channel-2-dependent Ca2+ signaling. Proc Natl Acad Sci U S A. 111:E4706
PMID: 25331892

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