RADA
Tocris Bioscience, part of Bio-Techne | Catalog # 6649
Orange-red TAMRA-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Key Product Details
Description
Orange-red TAMRA-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Alternative Names
TADA
Product Description
RADA is an orange-red TAMRA-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Suitable for prolonged exposure due to red-shift photophysical properties. Suitable for dSTORM and reSTORM. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~554/580 nm.This product is supplied as a TFA salt, the stoichiometry of which is batch dependant. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Scientific Data Images for RADA
Application of RADA.
Using FDAAs RADA and HADA (Catalog # 6647) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale: 5 μm.For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841
Optical Data
| Emission Color | Orange |
| λabs | 554 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95000 M-1cm-1 |
| Quantum Yield (φ) | 0.69 |
| Application | dSTORM, reSTORM |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
516.55
Formula
C28H28N4O6
Storage
Store at -20°C
Purity
≥98% (HPLC)
InChI Key
SMFYRQAHWDWBTD-AVHJOICASA-N
SMILES
N[C@@H](C(O)=O)CNC(C1=CC(C(C2=C(C=C(N(C)C)C=C2)O3)=C4C3=C/C(C=C4)=[N+](C)\C)=C(C=C1)C([O-])=O)=O.N[C@@H](C(O)=O)CNC(C5=CC(C([O-])=O)=C(C(C6=C(C=C(N(C)C)C=C6)O7)=C8C7=C/C(C=C8)=[N+](C)\C)C=C5)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 51.66 | 100 |
Preparing Stock Solutions
for RADA
The following data is based on the product molecular weight 516.55
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.94 mL | 9.68 mL | 19.36 mL |
| 5 mM | 0.39 mL | 1.94 mL | 3.87 mL |
| 10 mM | 0.19 mL | 0.97 mL | 1.94 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.39 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- García-Heredia Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 2018 PMID: 30198841
- Fenton Erratum: CozE is a member of the MreCD complex that directs cell elongation in Streptococcus pneumoniae. Nat.Microbiol. 2017 PMID: 28112724
- Hsu Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 2017 PMID: 28989665
Product Specific Notices for RADA
For research use only
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