HADA
Tocris Bioscience, part of Bio-Techne | Catalog # 6647
Blue fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Key Product Details
Description
Blue fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Product Description
HADA is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Results in strong peripheral and septal labeling of diverse bacterial cell populations without affecting growth rates. Displays mitochondrial outer membrane permeability. Excitation/emission λ ~405/450 nm.Scientific Data Images for HADA
Investigating mycobacteria cell wall formation using FDAA HADA.
Investigating mycobacteria cell wall formation using fluorescent D-amino acid HADA. Image showing FDAA HADA incorporation in log-phase growth of WT, delta L,D-transpeptidases (LDT) and delta L,D-transpeptidases (LDT) comp cells. Scale = 5 μm. Baranowski et al show FDAAs are incorporated asymmetrically by L,D-transpeptidases. For more information on this work and to request full protocols read Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. Elife 16 e37516 PMID: 30324906
Using FDAAs HADA and RADA to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time.
Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA (Catalog # 6649) chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale = 5 μm. For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841Optical Data
| Emission Color | Blue |
| λabs | 405 nm |
| λem | 450 nm |
| Extinction Coefficient (ε) | 36,700 M-1cm-1 |
| Application | Fluorescence Microscopy |
Spectra Viewer
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Product Specifications
Molecular Weight
328.71
Formula
C13H12N2O6.HCl
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl)carbonyl]amino]-D-alanine hydrocholoride
CAS Number
2253733-10-5
PubChem ID
137919867
InChI Key
PLVOEKQNBIXHDI-SBSPUUFOSA-N
SMILES
N[C@@H](C(O)=O)CNC(C1=CC(C=CC(O)=C2)=C2OC1=O)=O.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.87 | 100 |
Preparing Stock Solutions
for HADA
The following data is based on the product molecular weight 328.71
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.04 mL | 15.21 mL | 30.42 mL |
| 5 mM | 0.61 mL | 3.04 mL | 6.08 mL |
| 10 mM | 0.30 mL | 1.52 mL | 3.04 mL |
| 50 mM | 0.06 mL | 0.30 mL | 0.61 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Hsu Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 2017 PMID: 28989665
- Botella Distinct spatiotemporal dynamics of peptidoglycan synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis. Mbio. 2017 PMID: 28900018
- Kuru In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 2012 PMID: 23055266
Product Specific Notices for HADA
For research use only
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