Phalloidin-Janelia Fluor® 646
Tocris Bioscience, part of Bio-Techne | Catalog # 7201
Key Product Details
| Description: | Red-fluorescent F-actin probe. Binds and labels F-actin. Used for: F-actin labelling. Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED, flow cytometry |
| Alternative Names: | Phalloidin-JF646 |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 1266.51 |
Biological Activity for Phalloidin-Janelia Fluor® 646
Key information: Phalloidin-Janelia Fluor® 646 is a red fluorescent F-actin probe. It binds and labels specifically F-actin.
Used for: Phalloidin-Janelia Fluor® 646 can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.
Application: confocal microscopy, super-resolution microscopy (SRM) including dSTORM and STED and flow cytometry.
Properties and Photophysical Data: Phalloidin-Janelia Fluor® 646 is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Janelia Fluor® 646 (Cat. No. 6148). Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Phalloidin-Janelia Fluor® 646
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Application | Confocal Imaging, dSTORM, STED, Flow Cytometry |
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Application of Phalloidin-Janelia Fluor® 646. HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München. |
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Application of Phalloidin-Janelia Fluor® 646. HeLa cells were fixed for 10 minutes with 4% formaldehyde in PBS and permeabilized with PBS/0.25% Triton X-100 for 6 minutes. An additional post-fixation step was performed twice with 1% formaldehyde in PBS followed by 3 washes with PBS. Phalloidin Janelia Fluor® 646 was prepared as a 10 μM stock solution diluted in methanol and diluted to a working concentration of 20 nM in PBS. Stained cells were imaged with a Leica SP8X WLL upright confocal microscope. Image kindly provided by Brigitte Bergner, Steffen Dietzel, Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München. |
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