M2 Macrophage Flow Cytometry Panel
R&D Systems, part of Bio-Techne | Catalog # FMC-P-007
M2 Macrophage Flow Cytometry Panel
Key Product Details
Features
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Species
Human
Conjugate
Multiple
Scientific Data Images for M2 Macrophage Flow Cytometry Panel
Multicolor flow cytometry panel to identify M2 Macrophages.
Monocytes were isolated from PBMCs via adherence depletion for 5 hours. Non-adherent cells were removed. Adherent cells were incubated for 6 days in C3 +10% huAB media with 50 ng/ml Recombinant Human M-CSF (216-MC), media and cytokines are refreshed on day 3. For M2 polarization, 24 hours prior to staining, cells are incubated with 50 ng/ml human M-CSF (216-MC), 20 ng/ml Recombinant Human IL-4 (204-IL), and 20 ng/ml Recombinant Human IL-13 (213-ILB). Cells were stained with Anti-Human CD3 Alexa Fluor® 405, CD20 Alexa Fluor 405, CD56 Alexa Fluor 700, CD206 Alexa Fluor 488, CD163 PerCP, CD204 PE, and VEGF APC. Cells were previously gated on Live CD3-CD20-CD56-. Find Staining Protocol Here.Formulation, Preparation, and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Flow Cytometry Panel
Long Name
Flow Cytometry Panel
Additional Flow Cytometry Panel Products
Product Documents for M2 Macrophage Flow Cytometry Panel
Product Specific Notices for M2 Macrophage Flow Cytometry Panel
For research use only
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