SARS-CoV-2 Spike RBD - Omicron Variant, KP.3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48428

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Recombinant Protein (100 ul)

Sensitivity

2.07 pg/mL (example only; lot dependent)

Assay Range

39.06-2500 pg/mL (example only; lot dependent)

View all Spike RBD ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

SARS-CoV-2

Description

Solid Phase sandwich ELISA for the quantitative determination of SARS-CoV-2 Spike RBD Omicron Variant, KP.3

Precision

Intra-Assay Precision (Precision within an assay) CV % < 3.4%

Inter-Assay Precision (Precision between assays) CV % < 5.3%

Recovery

Recovery

95-105%

Kit Contents

  • 96 well microplate coated with Capture Antibody
  • Color Reagent A
  • Color Reagent B
  • Detection Antibody conjugated to HRP
  • Dilution Buffer Concentrate
  • Standards
  • Stop Solution
  • Wash Buffer Concentration

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Spike RBD

The coronavirus Spike protein receptor binding domain (RBD) resides within the S1 subunit and is responsible for binding to host cell receptors and initiating viral infection. Past coronaviruses SARS and MERS along with the global pandemic caused by SARS-CoV-2 have sparked great interest and scientific discovery leading to new vaccines and drug development. At the heart of coronavirus biology lies the Spike protein and its receptor binding domain. The Spike RBD is a 26 kDa domain consisting of a twisted five-stranded antiparallel beta sheet and sits at the apex of each Spike protein monomer. The Spike RBD is flexible thanks to a hinge region that allows for conformational changes that expose (up or open conformation) or hide (down or closed conformation) its receptor contacts. For SARS-CoV-2, the Spike RBD recognizes and tightly binds to the human receptor ACE-2. In the closely related MERS coronavirus, human DPPIV/CD26 acts as the receptor.

Long Name

Spike Receptor Binding Domain

Additional Spike RBD Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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