Rat ROBO1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY1749
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
156-10000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Rat
Rat ROBO1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Rat ROBO1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant rat ROBO1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Rat ROBO1 DuoSet ELISA
Rat ROBO1 ELISA Standard Curve
Kit Contents for Rat ROBO1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ROBO1
ROBO1 is an axon guidance molecule that is expressed on pioneer axons in the neural tube and proliferative zones of the cortical plate. It plays an important role in axon pathfinding and tract development as well as in neuronal proliferation and dendrite branching. It is restricted to axonal growth cones that do not cross the midline and to midline-crossing axons only after they cross. ROBO1 binds to Slit1 and Slit2, leading to growth cone collapse and axonal branching. It also regulates Netrin-1 and Semaphorin 3A and 3F responsiveness through its ability to form in cis complexes with Neuropilin-1, Neuropilin-2, Plexin A1, DCC, and FLRT3. ROBO1-Slit2 interactions restrict branching morphogenesis in developing ductal epithelium, suppress tumorigenesis, enhance VEGF-induced angiogenesis, and enhance CXCL12 induced T cell chemotaxis by associating with CXCR4.
Long Name
Roundabout Homolog 1
Alternate Names
DUTT1
Gene Symbol
ROBO1
Additional ROBO1 Products
Product Documents for Rat ROBO1 DuoSet ELISA
Product Specific Notices for Rat ROBO1 DuoSet ELISA
For research use only
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