Porcine IL-8/CXCL8 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # P8000
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL)
Sensitivity
6.7 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Porcine IL-8/CXCL8 Quantikine ELISA Kit
The Quantikine Porcine IL-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-8 in cell culture supernates and serum. It utilizes E. coli-expressed recombinant porcine IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately measure or quantify the recombinant porcine IL-8. Results obtained using natural porcine IL-8 show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-8.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Porcine
Specificity
Natural and recombinant porcine IL-8
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 97 | 748 | 1607 | 106 | 717 | 1641 |
| Standard Deviation | 9.4 | 64 | 106 | 9.0 | 49 | 108 |
| CV% | 9.7 | 8.6 | 6.6 | 8.5 | 6.8 | 6.6 |
Recovery for Porcine IL-8/CXCL8 Quantikine ELISA Kit
The recovery of porcine IL-8 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 108 | 80-120 |
| Serum (n=7) | 91 | 80-100 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of porcine IL-8 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Porcine IL-8/CXCL8 Quantikine ELISA Kit
Porcine CXCL8/IL-8 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-8/CXCL8
Interleukin-8 (IL-8), also known as CXCL8, is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. IL-8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. IL-8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. IL-8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents IL-8 interaction with CXCR1.
Long Name
Interleukin 8
Alternate Names
CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1
Gene Symbol
CXCL8
Additional IL-8/CXCL8 Products
Product Documents for Porcine IL-8/CXCL8 Quantikine ELISA Kit
Product Specific Notices for Porcine IL-8/CXCL8 Quantikine ELISA Kit
For research use only
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.