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Rat NF-L ELISA Kit (Colorimetric)

Catalog # NBP2-81185 | Novus Biologicals a Bio-Techne Brand
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NBP2-81185

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat NF-L in samples. No significant cross-reactivity or interference between Rat NF-L and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.36%

Inter-Assay Precision (Precision between assays) CV% < 4.4%

Recovery for Rat NF-L ELISA Kit (Colorimetric)

Recovery

87-102%

Linearity

Rat NF-L ELISA Kit (Colorimetric)

Scientific Data Images for Rat NF-L ELISA Kit (Colorimetric)

Rat NF-L ELISA Kit (Colorimetric) [NBP2-81185] - Typical standard curve. The provided standard curve is for reference only, as the OD values may vary according to the conditions of the actual experiment. The standard curve should be established for each test.

Kit Contents for Rat NF-L ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate(Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: NF-L

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Gene Symbol

NEFL

Product Documents for Rat NF-L ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat NF-L ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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