Mouse TWEAK/TNFSF12 DuoSet ELISA

Name: Mouse TWEAK/TNFSF12 DuoSet ELISA
Brand: R&D Systems
SKU: DY1237

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY1237

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	15.6 - 1,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse TWEAK/TNFSF12 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Mouse TWEAK/TNFSF12 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse TWEAK / TNFSF12. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Mouse TWEAK/TNFSF12 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse TWEAK/TNFSF12 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

Scientific Data Examples for Mouse TWEAK/TNFSF12 DuoSet ELISA

	Mouse TWEAK / TNFSF12 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TWEAK/TNFSF12

TWEAK is a type II transmembrane protein belonging to the TNF superfamily. The extracellular domains of human and mouse TWEAK share 89% amino acid sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecule by proteolytic cleavage after Arg 93, suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells.

Item	Value
Long Name	TNF-like and Weak Inducer of Apoptosis
Entrez Gene IDs	8742 (Human); 21944 (Mouse); 360548 (Rat)
Alternate Names	APO3 ligand, APO3LMGC20669, DR3LGTWEAKAPO3/DR3 ligand, MGC129581, TNF-related weak inducer of apoptosis, TNFSF12, TWEAK, tumor necrosis factor (ligand) superfamily, member 12, tumor necrosis factor ligand superfamily member 12

Citations for Mouse TWEAK/TNFSF12 DuoSet ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse TWEAK/TNFSF12 DuoSet ELISA include:

Species: Mouse
Sample Types: Serum

K Browne et al. (2021), Lupus-prone B6.Nba2 male and female mice display anti-DWEYS reactivity and a neuropsychiatric phenotype Brain, Behavior, and Immunity, 0(0).
PMID: 33607233
Species: Mouse
Sample Types: Serum

KS Tummala et al. (2017), Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells Cell Rep, 19(3):584-600.
PMID: 28423321
Species: Mouse
Sample Types: Tissue Homogenates

Al-Sawaf O et al. (2014), Nrf2 protects against TWEAK-mediated skeletal muscle wasting. Sci Rep, 4(0):3625.
PMID: 24406502

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