Mouse/Rat PDGF-BB DuoSet ELISA

Name: Mouse/Rat PDGF-BB DuoSet ELISA
Brand: R&D Systems
SKU: DY8464-05
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY8464-05

Citations (2)

(1)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse/Rat PDGF-BB DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Mouse/Rat PDGF-BB DuoSet ELISA

Mouse/Rat PDGF-BB DuoSet ELISA, 5 Plate

Product Specifications for Mouse/Rat PDGF-BB DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse, Rat
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse/Rat PDGF-BB DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Mouse/Rat PDGF-BB DuoSet ELISA

	Mouse / Rat PDGF-BB ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-BB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Item	Value
Long Name	Platelet-derived Growth Factor BB
Entrez Gene IDs	5155 (Human); 24628 (Rat)
Alternate Names	PDGF-BB, PDGFBB

Citations for Mouse/Rat PDGF-BB DuoSet ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat PDGF-BB DuoSet ELISA include:

Species: Mouse
Sample Types: Tissue Lysate

Y Zhang et al. (2022), Platelet-Derived PDGFB Promotes Recruitment of Cancer-Associated Fibroblasts, Deposition of Extracellular Matrix and Tgfbeta Signaling in the Tumor Microenvironment Cancers, 14(8).
PMID: 35454853
Species: Mouse
Sample Types: Cell Culture Supernates

T Castro-Dop et al. (2020), GM-CSF Calibrates Macrophage Defense and Wound Healing Programs during Intestinal Infection and Inflammation Cell Rep, 32(1):107857.
PMID: 32640223

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Mouse/Rat PDGF-BB DuoSet ELISA, 5 Plate

Name: Anonymous

Sample Tested: Lung tissue

Verified Customer | Posted 05/02/2017

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Product Documents for Mouse/Rat PDGF-BB DuoSet ELISA

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