Mouse/Rat IGF-I/IGF-1 DuoSet ELISA

Name: Mouse/Rat IGF-I/IGF-1 DuoSet ELISA
Brand: R&D Systems
SKU: DY791
Rating: 5 (2 reviews)

Catalog # DY791 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (20)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	31.2 - 2,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse/Rat IGF-I/IGF-1 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA

Technical Data
Citations (20) / Reviews (2)

Summary for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IGF-I. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse, Rat
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 3 (5 plates): (Catalog # DY009) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 3.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY004), or 5% Tween 20 in PBS, 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA

	Mouse / Rat IGF-I ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I/IGF-1

Insulin-like Growth Factor 1 (IGF-1), also known as somatomedin C, is a member of the insulin superfamily. It was originally discovered as a mediator of growth hormone actions on somatic cell growth, but has also been shown to be an important regulator of cell metabolism, differentiation and survival. IGF-1 is synthesized as a preproprotein that is proteolytically cleaved to generate the mature protein linked by three disulfide bonds. Mature IGF-1 is highly conserved among large mammals, with 100% sequence identity between the human, bovine, porcine, equine, and canine proteins.

Mature mouse IGF-1 is a non-glycosylated, 70 amino acid (aa) secreted polypeptide that is derived from either a 153 aa or a 159 aa preproprotein. It shares 99% and 94% aa sequence identity with rat and human IGF-1, respectively. IGF-1 is synthesized in the liver and other tissues. It is found in blood and other body fluids as a complex with specific high affinity IGF binding proteins (IGFBP-1 to -6). The IGFBPs are expressed in specific patterns during development. They are modulators of IGF actions, which control IGF bioavailability to specific cell-surface receptors. Their functions are further regulated by IGFBP proteases, which proteolytically cleave the IGFBPs to lower the affinity with which they bind IGFs and increase IGF bioavailability. Some IGFBPs also have IGF-Independent effects on cell functions. IGF-Independent circulates primarily as a ternary complex with IGFBP-3 or IGFBP-5 and the acid-labile subunit (ALS). Some IGF-1 is also present in binary complexes with other IGFBPs. Whereas the ternary complexes are generally restricted to the vasculature, the binary complexes freely enter the tissues.

IGF-1 actions are mediated by two ubiquitously expressed receptor tyrosine kinases: IGF-I R and Insulin R/CD220. IGF-I R and Insulin R are disulfide-linked heterotetrameric complexes that consist of two alpha and two beta subunits. For both of these receptors, the prepro proteins are cleaved to produce extracellular alpha subunits which contain a cysteine-rich region and ligand-binding fibronectin type III (FN-III) domains, and beta subunits which contain an extracellular FN-III domain, transmembrane, and cytoplasmic tyrosine kinase domains. A hybrid complex containing one IGF-I R and one Insulin R also serves as a functional high affinity receptor for IGF-1. IGF-I R-Insulin R hybrids respond primarily to IGF-1, potentially downregulating the cellular response to Insulin. IGF signaling is also modulated by IGF binding proteins and the scavenger receptor, IGF-I I R.

Item	Value
Long Name	Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs	3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names	IBP1, IGF-1, IGF-I, IGF-IA, IGF-IB, IGF1, IGF1A, IGFI, MGF, Mechano growth factor, Somatomedin A, Somatomedin C, insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor I, insulin-like growth factor IA, insulin-like growth factor IB, somatomedin-C

Citations for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA (20)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA include:

Species: Mouse
Sample Types: Plasma

E Butti et al. (2022-12-08), Neural precursor cells tune striatal connectivity through the release of IGFBPL1 Nature Communications, 2022-113(1):7579.
PMID: 36482070
Species: Rat
Sample Types: Urine

AS Naik et al. (2021-10-01), Critical timing of ACEi initiation prevents compensatory glomerular hypertrophy in the remaining single kidney Scientific Reports, 2021-111(1):19605.
PMID: 34599260
Species: Mouse
Sample Types: Serum

MD Rogowska et al. (2021-09-01), Recovery of the maternal skeleton after lactation is impaired by advanced maternal age but not by reduced IGF availability in the mouse PLoS ONE, 2021-016(9):e0256906.
PMID: 34469481
Species: Rat
Sample Types: Serum

S Keshvari et al. (2021-06-03), CSF1R-dependent macrophages control postnatal somatic growth and organ maturation PloS Genetics, 2021-017(6):e1009605.
PMID: 34081701
Species: Mouse
Sample Types: Tissue Homogenates

M Hong et al. (2021-03-20), Magnoliae Cortex Alleviates Muscle Wasting by Modulating M2 Macrophages in a Cisplatin-Induced Sarcopenia Mouse Model International Journal of Molecular Sciences, 2021-022(6).
PMID: 33804803
Species: Mouse
Sample Types: Serum

J Xu et al. (2020-10-30), Embryonic stem cell-derived mesenchymal stem cells promote colon epithelial integrity and regeneration by elevating circulating IGF-1 in colitis mice Theranostics, 2020-110(26):12204-12222.
PMID: 33204338
Species: Mouse
Sample Types: Tissue Homogenates

A Wolf et al. (2020-06-01), The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye Nat Commun, 2020-011(1):2709.
PMID: 32483169
Species: Mouse
Sample Types: Serum

X Niu et al. (2019-11-13), Proteasome Activation by Insulin-like Growth Factor-1/Nuclear Factor Erythroid 2-related Factor 2 Signaling Promotes Exercise-induced Neurogenesis Stem Cells, 2019-10(0).
PMID: 31648402
Species: Mouse
Sample Types: Serum

F Ascenzi et al. (2019-04-05), Effects of IGF-1 isoforms on muscle growth and sarcopenia Aging Cell, 2019-00(0):e12954.
PMID: 30953403
Species: Mouse
Sample Types: Serum

KME Andersson et al. (2018-12-03), Inflammation in the hippocampus affects IGF1 receptor signaling and contributes to neurological sequelae in rheumatoid arthritis Proc. Natl. Acad. Sci. U.S.A., 2018-10(0).
PMID: 30509997
Species: Mouse
Sample Types: Cell Culture Supernates

V Giambra et al. (2018-09-27), Epigenetic Restoration of Fetal-like IGF1 Signaling Inhibits Leukemia Stem Cell Activity Cell Stem Cell, 2018-00(0).
PMID: 30269902
Species: Mouse
Sample Types: Cell Culture Supernates

L R Batista-Si (2016-06-10), Mycobacterium leprae-induced Insulin-like Growth Factor I attenuates antimicrobial mechanisms, promoting bacterial survival in macrophages Sci Rep, 2016-06(0):27632.
PMID: 27282338
Species: Mouse
Sample Types: Serum

Liu et al. (2014-03-18), Hypothalamic gene transfer of BDNF inhibits breast cancer progression and metastasis in middle age obese mice. Mol Ther, 2014-022(7):1275-84.
PMID: 24637454
Species: Mouse
Sample Types: Tissue Homogenates

Hansen et al. (2013-11-19), The lung inflammation and skeletal muscle wasting induced by subchronic cigarette smoke exposure are not altered by a high-fat diet in mice. PLoS ONE, 2013-18(11):e80471.
PMID: 24260397
Species: Mouse
Sample Types: Tissue Homogenates

Sato A et al. (2012-04-07), Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation, 2012-09(0):65.
PMID: 22483094
Species: Mouse
Sample Types: Plasma

Kelly-Spratt KS et al. (2011-05-12), Plasma proteome profiles associated with inflammation, angiogenesis, and cancer. PLoS ONE, 2011-06(5):e19721.
PMID: 21589862
Species: Mouse
Sample Types: Serum

Childs GV et al. (2010-11-17), The somatotrope as a metabolic sensor: deletion of leptin receptors causes obesity. Endocrinology, 2010-1152(1):69-81.
PMID: 21084451
Species: Mouse
Sample Types: Cell Culture Supernates

Wanderling S et al. (2007-07-18), GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion. Mol. Biol. Cell, 2007-018(10):3764-75.
PMID: 17634284
Species: Mouse
Sample Types: Cell Culture Supernates

Xiao Q et al. (2007-06-07), Mutant SOD1(G93A) microglia are more neurotoxic relative to wild-type microglia. J. Neurochem., 2007-0102(6):2008-19.
PMID: 17555556
Species: Rat
Sample Types: Cell Culture Supernates

Zhao W et al. (2006-10-02), Protective effects of an anti-inflammatory cytokine, interleukin-4, on motoneuron toxicity induced by activated microglia. J. Neurochem., 2006-199(4):1176-87.
PMID: 17018025

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Customer Reviews for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA (2)

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Mouse/Rat IGF-I DuoSet ELISA

Name: Anonymous

Sample Tested: Serum

Verified Customer | Posted 11/06/2018
Mouse/Rat IGF-I DuoSet ELISA

Name: Leslie Priddy

Sample Tested: Serum

Verified Customer | Posted 03/31/2018

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Product Documents for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA

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