Mouse Park7/DJ-1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY8136-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.313-20 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse
Mouse Park7/DJ-1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Mouse Park7/DJ-1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Park7 / DJ-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse Park7/DJ-1 DuoSet ELISA
Mouse Park7 / DJ-1 ELISA Standard Curve
Kit Contents for Mouse Park7/DJ-1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Park7/DJ-1
Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson's disease.
Long Name
Parkinson Disease 7
Alternate Names
DJ-1, DJ1
Gene Symbol
PARK7
Additional Park7/DJ-1 Products
Product Documents for Mouse Park7/DJ-1 DuoSet ELISA
Product Specific Notices for Mouse Park7/DJ-1 DuoSet ELISA
For research use only
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