Mouse IL-6R alpha Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MR600
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.07 ng/mL
Assay Range
0.188-12 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Product Summary for Mouse IL-6R alpha Quantikine ELISA Kit
The Quantikine Mouse IL-6 R alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-6 R alpha levels in cell culture supernates, serum, and heparin plasma. It contains NS0-expressed recombinant mouse IL-6 R alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-6 R alpha accurately. Results obtained using natural mouse IL-6 R alpha showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6 R alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-6 R alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.456 | 1.07 | 2.94 | 0.474 | 1.08 | 2.82 |
| Standard Deviation | 0.036 | 0.039 | 0.148 | 0.060 | 0.080 | 0.200 |
| CV% | 7.9 | 3.6 | 5.0 | 12.7 | 7.4 | 7.1 |
Recovery for Mouse IL-6R alpha Quantikine ELISA Kit
The recovery of mouse IL-6 R alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 90-110 |
| Heparin Plasma (n=4) | 99 | 92-106 |
| Serum (n=4) | 102 | 93-110 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 R alpha were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse IL-6R alpha Quantikine ELISA Kit
Mouse IL-6 R alpha ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6R alpha
IL-6R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.
Long Name
Interleukin 6 Receptor alpha
Alternate Names
CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha
Gene Symbol
IL6R
Additional IL-6R alpha Products
Product Documents for Mouse IL-6R alpha Quantikine ELISA Kit
Product Specific Notices for Mouse IL-6R alpha Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.