Mouse IL-20 DuoSet ELISA

Name: Mouse IL-20 DuoSet ELISA
Brand: R&D Systems
SKU: DY1204

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY1204

Citations (6)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	31.2 - 2,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse IL-20 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Mouse IL-20 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-20. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Mouse IL-20 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse IL-20 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

Scientific Data Examples for Mouse IL-20 DuoSet ELISA

	Mouse IL-20 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-20

Mature human and mouse IL-20 is a 152 amino acid protein that is not likely glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor-expressing cells.

Item	Value
Long Name	Interleukin 20
Entrez Gene IDs	50604 (Human); 58181 (Mouse)
Alternate Names	Cytokine Zcyto10, IL-20, IL-20ZCYTO10four alpha helix cytokine, IL10D, IL20, MGC96907, interleukin 20, interleukin-20

Citations for Mouse IL-20 DuoSet ELISA (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-20 DuoSet ELISA include:

Species: Mouse
Sample Types: Tears

HH Wang et al. (2022), Interleukin-20 is involved in dry eye disease and is a potential therapeutic target Journal of Biomedical Science, 29(1):36.
PMID: 35681232
Species: Mouse
Sample Types: Tissue Homogenates

JR Dayton et al. (2021), Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation Frontiers in Cellular Neuroscience, 15(0):683687.
PMID: 34557075
Species: Mouse
Sample Types: BALF

M Le Roux et al. (2021), IL-20 Cytokines Are Involved in Epithelial Lesions Associated with Virus-Induced COPD Exacerbation in Mice Biomedicines, 9(12).
PMID: 34944654
Species: Mouse
Sample Types: Serum

SW Lu et al. (2020), IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models Nat Commun, 11(1):4611.
PMID: 32929072
Species: Mouse
Sample Types: Tissue Homogenates

Alan R Burns (2016), IL-20 promotes epithelial healing of the injured mouse cornea Exp. Eye Res., 154(0):22-29.
PMID: 27818315
Species: Mouse
Sample Types: Tissue Homogenates

Blumberg H et al. (2010), IL-1RL2 and its ligands contribute to the cytokine network in psoriasis. J. Immunol., 185(7):4354-62.
PMID: 20833839

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Showing 1 - 6 of 6 citations Showing All

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