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Mouse IFN-beta DuoSet ELISA Best Seller

Catalog # DY8234-05 | R&D Systems, Inc. a Bio-Techne Brand
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DY8234-05

Ancillary Products Available

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

12.5-800 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse IFN-beta DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Product Summary for Mouse IFN-beta DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Interferon beta (IFN-β). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Label

HRP

Scientific Data Images for Mouse IFN-beta DuoSet ELISA

Mouse IFN-beta ELISA Standard Curve

Mouse IFN-beta ELISA Standard Curve

Kit Contents for Mouse IFN-beta DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name

Interferon beta

Alternate Names

IFB, IFF, IFNB, IFNB1, IFNbeta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Gene Symbol

IFNB1

Product Documents for Mouse IFN-beta DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IFN-beta DuoSet ELISA

For research use only

Citations for Mouse IFN-beta DuoSet ELISA (31)

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Customer Reviews for Mouse IFN-beta DuoSet ELISA (6)

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  • Mouse IFN-beta DuoSet ELISA
    Name: Anonymous
    Sample Tested: HEK293 cell line and 293T human embryonic kidney cell line
    Verified Customer | Posted 06/23/2023
  • Mouse IFN-beta DuoSet ELISA
    Name: Anonymous
    Sample Tested: Mouse lymphocytes
    Verified Customer | Posted 12/14/2020
    Mouse IFN-beta DuoSet ELISA DY8234-05
  • Mouse IFN-beta DuoSet ELISA
    Name: Anonymous
    Sample Tested: mouse microglia
    Verified Customer | Posted 12/12/2019
    Mouse IFN-beta DuoSet ELISA DY8234-05
  • Mouse IFN-beta DuoSet ELISA
    Name: Anonymous
    Sample Tested: Mouse spleenocytes
    Verified Customer | Posted 09/11/2019
  • Mouse IFN-beta DuoSet ELISA
    Name: Kim Han
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 01/03/2019
  • Mouse IFN-beta DuoSet ELISA
    Name: Anonymous
    Sample Tested: Mouse and Spinal cord tissue
    Verified Customer | Posted 09/25/2018
    Mouse IFN-beta DuoSet ELISA DY8234-05

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