Mouse Galectin-1 DuoSet ELISA

Name: Mouse Galectin-1 DuoSet ELISA
Brand: R&D Systems
SKU: DY1245

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY1245

Citations (10)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	125 - 8,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse Galectin-1 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Mouse Galectin-1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Galectin-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Mouse Galectin-1 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse Galectin-1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Mouse Galectin-1 DuoSet ELISA

	Mouse Galectin-1 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Item	Value
Entrez Gene IDs	3956 (Human); 16852 (Mouse)
Alternate Names	14 kDa laminin-binding protein, 14 kDa lectin, BHL, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, GAL1, GBP, Galaptin, Galectin-1, Galectin1, HBL, HLBP14, HPL, L-14, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, gal-1, galectin 1, lectin, galactoside-binding, soluble, 1

Citations for Mouse Galectin-1 DuoSet ELISA (10)

Showing 1 - 10 of 10 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Galectin-1 DuoSet ELISA include:

Species: Mouse
Sample Types: Plasma

SO Vasudevan et al. (2022), A TLR4-independent critical role for CD14 in intracellular LPS sensing Cell Reports, 39(5):110755.
PMID: 35508125
Species: Mouse
Sample Types: Plasma

SS Kim et al. (2022), A Novel P53 Nanomedicine Reduces Immunosuppression and Augments Anti-PD-1 Therapy for Non-Small Cell Lung Cancer in Syngeneic Mouse Models Cells, 11(21).
PMID: 36359830
P Kumari et al. (2021), Hierarchical cell-type-specific functions of caspase-11 in LPS shock and antibacterial host defense Cell Reports, 35(3):109012.
PMID: 33882312
Species: Mouse
Sample Types: Peritoneal Cavity Lavage Fluid

HL Law et al. (2020), A Pro-resolving Role for Galectin-1 in Acute Inflammation Front Pharmacol, 11(0):274.
PMID: 32265698
Species: Mouse
Sample Types: Cell Culture Supernates

JM Lykken et al. (2016), Galectin-1 drives lymphoma CD20 immunotherapy resistance: validation of a preclinical system to identify resistance mechanisms Blood, 127(15):1886-95.
PMID: 26888257
Species: Mouse
Sample Types: Serum

Van Ry P et al. (2015), Galectin-1 Protein Therapy Prevents Pathology and Improves Muscle Function in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther, 23(8):1285-97.
PMID: 26050991
Species: Mouse
Sample Types: Plasma

Al-Salam S et al. (2014), Galectin-1 in early acute myocardial infarction. PLoS ONE, 9(1):e86994.
PMID: 24498007
Species: Mouse
Sample Types: Serum

Xie et al. (2013), Lupus-prone strains vary in susceptibility to antibody-mediated end organ disease. Genes Immun, 14(3):170-8.
PMID: 23392277
Species: Mouse
Sample Types: Plasma

Pitteri SJ et al. (2011), Tumor microenvironment-derived proteins dominate the plasma proteome response during breast cancer induction and progression. Cancer Res., 71(15):5090-100.
PMID: 21653680
Species: Mouse
Sample Types: Cell Culture Supernates

Tsai CM et al. (2008), Galectin-1 promotes immunoglobulin production during plasma cell differentiation. J. Immunol., 181(7):4570-9.
PMID: 18802059

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