Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49789

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Lactate Dehydrogenase A/LDHA ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes RatLactate Dehydrogenase A/LDHA in samples. No significant cross-reactivity or interference between Rat LDHA and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

Scientific Data Images for Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

ELISA: Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric) [NBP3-49789]

Standard Curve Reference.

Kit Contents for Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lactate Dehydrogenase A/LDHA

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Alternate Names

LDH1, LDHA, LDHM

Gene Symbol

LDHA

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Lactate Dehydrogenase A/LDHA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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