Human VE-Cadherin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCADV0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.236 ng/mL
Assay Range
1.56-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human VE-Cadherin Quantikine ELISA Kit
The Quantikine Human VE-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VE-Cadherin in cell culture supernates, serum, and plasma. It contains NS0-derived recombinant human VE-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VE-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VE-Cadherin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human VE-Cadherin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 13.4 | 34.6 | 65.4 | 12.8 | 31.8 | 64.5 |
| Standard Deviation | 0.68 | 1.63 | 2.05 | 0.92 | 1.64 | 2.86 |
| CV% | 5.1 | 4.7 | 3.1 | 7.2 | 5.1 | 4.4 |
Recovery for Human VE-Cadherin Quantikine ELISA Kit
The recovery of VE-Cadherin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 94-108 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of VE-Cadherin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human VE-Cadherin Quantikine ELISA Kit
Human VE-Cadherin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VE-Cadherin
VE-Cadherin, also known as Cadherin-5 and CD144, is a member of the atypical/type II subgroup of Cadherin homophilic adhesion proteins. A 90 kDa portion of the VE-Cadherin ECD can be shed in a metalloproteinase-dependent mechanism and may function as a VE-Cadherin antagonist. VE-Cadherin is expressed on the surface of vascular endothelial cells (EC) from early in embryogenesis through adulthood as well as in the placenta, on hematopoietic cell progenitors, and on a subpopulation of hematopoietic stem cells. It is a major protein component of adherens junctions between cells of the endothelium. VE-Cadherin supports the extension and stabilization of existing vascular sprouts. VE-Cadherin also regulates or is regulated by VEGF R2, type I and type II TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin. Elevated levels of soluble VE-Cadherin are found in the serum of myocardial infarction, angina pectoris, rheumatoid arthritis, and colorectal cancer patients.
Long Name
Vascular Endothelium Cadherin
Alternate Names
Cadherin-5, CD144, CDH5, VECadherin
Gene Symbol
CDH5
Additional VE-Cadherin Products
Product Documents for Human VE-Cadherin Quantikine ELISA Kit
Product Specific Notices for Human VE-Cadherin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.