Human Total p53 DuoSet IC ELISA

Name: Human Total p53 DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC1043-2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DYC1043-2

Citations (6)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	125 - 8,000 pg/mL
Sample Types:	Cell Lysates
Human Total p53 DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

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Summary for Human Total p53 DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total p53 in cell lysates. An immobilized capture antibody specific for p53 binds both phosphorylated and unphosphorylated p53. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human Total p53 DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Total p53 DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human Total p53 DuoSet IC ELISA

	Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: p53

p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.

Item	Value
Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names	Antigen NY-CO-13, BCC7, FLJ92943, LFS1, LFS1TRP53, P53cellular tumor antigen p53, Phosphoprotein p53, TP53, TRP53, Tumor suppressor p53, p53, p53 tumor suppressor, transformation-related protein 53, tumor protein p53

Citations for Human Total p53 DuoSet IC ELISA (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Total p53 DuoSet IC ELISA include:

Species: Human
Sample Types: Cell Lysates

A Verma et al. (2020), Loss of Estrogen Receptors is Associated with Increased Tumor Aggression in Laryngeal Squamous Cell Carcinoma Sci Rep, 10(1):4227.
PMID: 32144339
Species: Porcine
Sample Types: Plasma

T Huang et al. (2018), Regulators of Salmonella-host interaction identified by peripheral blood transcriptome profiling: roles of TGFB1 and TRP53 in intracellular Salmonella replication in pigs Vet. Res., 49(1):121.
PMID: 30541630
Species: Human
Sample Types: Cell Lysates

José M Pingarrón (2016), Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers Biosensors (Basel), 6(4).
PMID: 27879639
Species: Human
Sample Types: Cell Lysates

Abdullah A et al. (2015), Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor positive breast cancer MCF-7 cells. Int J Mol Sci, 16(2):3528-36.
PMID: 25664859
Eckstein N et al. (2007), Epidermal growth factor receptor pathway analysis identifies amphiregulin as a key factor for cisplatin resistance of human breast cancer cells. J. Biol. Chem., 283(2):739-50.
PMID: 17942395
Species: Complex Species Category
Sample Types: Cell Culture Supernates

Bhuyan S et al. (0), Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense. Front Immuno13(0):933329.
PMID: 36248858

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Showing 1 - 6 of 6 citations Showing All

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