Human TFF2 DuoSet ELISA

Name: Human TFF2 DuoSet ELISA
Brand: R&D Systems
SKU: DY4077

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY4077

Citations (3)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	7.81 - 500 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human TFF2 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human TFF2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TFF2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human TFF2 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human TFF2 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human TFF2 DuoSet ELISA

	Human TFF2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TFF2

The trefoil factor (TFF) family contains three secreted, protease-resistant polypeptides that help to maintain gastrointestinal system integrity. Family members are 7-12 kDa in size, and contain a 3-disulfide bond generated trefoil structure that resembles a meadow clover. TFF1, 2 and 3 are produced in different areas of the digestive tract, and promote restitution, or the resealing of superficial wounds after injury.

Item	Value
Long Name	Trefoil Factor 2
Entrez Gene IDs	7032 (Human); 21785 (Mouse); 116592 (Rat)
Alternate Names	SML1, SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1, SP, Spasmolysin, Spasmolytic Polypeptide, TFF2, trefoil factor 2

Citations for Human TFF2 DuoSet ELISA (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human TFF2 DuoSet ELISA include:

Species: Human
Sample Types: Serum

B Adewale et al. (2021), Parasitic helminth infections in humans modulate Trefoil Factor levels in a manner dependent on the species of parasite and age of the host PloS Neglected Tropical Diseases, 15(10):e0009550.
PMID: 34662329
Species: Human
Sample Types: Serum

R Jahan et al. (2019), Trefoil factor(s) and CA19.9: A promising panel for early detection of pancreatic cancer EBioMedicine, 0(0).
PMID: 30956167
Species: Human
Sample Types: Serum

D Lebherz-Ei et al. (2017), Increased trefoil factor 2 levels in patients with chronic kidney disease PLoS ONE, 12(3):e0174551.
PMID: 28355260

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