Human sVEGFR2/KDR DuoSet ELISA

Name: Human sVEGFR2/KDR DuoSet ELISA
Brand: R&D Systems
SKU: DY357
Price: 745 USD

Catalog # DY357 | R&D Systems, Inc. a Bio-Techne Brand

Citations (8)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	31.2 - 2,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human sVEGFR2/KDR DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Human sVEGFR2/KDR DuoSet ELISA

Summary for Human sVEGFR2/KDR DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VEGF R2/KDR. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human sVEGFR2/KDR DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human sVEGFR2/KDR DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

Normal Goat Serum: (Catalog # DY005)

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Normal Goat Serum: (Catalog # DY005)

Scientific Data Examples for Human sVEGFR2/KDR DuoSet ELISA

	Human VEGF R2 / KDR / Flk-1 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR2/KDR/Flk-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Item	Value
Long Name	Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
Alternate Names	CD309, CD309 antigen, EC 2.7.10, EC 2.7.10.1, FLK1tyrosine kinase growth factor receptor, Fetal liver kinase 1, Flk-1, Flk1, KDR, KRD1, Kinase insert domain receptor, Ly73, Protein-tyrosine kinase receptor flk-1, VEGF R2, VEGFR, VEGFR-2, VEGFR2, fetal liver kinase-1, kinase insert domain receptor (a type III receptor tyrosine kinase), soluble VEGFR2, vascular endothelial growth factor receptor 2

Citations for Human sVEGFR2/KDR DuoSet ELISA (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human sVEGFR2/KDR DuoSet ELISA include:

Species: Human
Sample Types: Plasma

FDD Nunes et al. (2022-12-09), The Association of Biochemical and Genetic Biomarkers in VEGF Pathway with Depression Pharmaceutics, 2022-114(12).
PMID: 36559251
Species: Human
Sample Types: Vitreous Humor

I Wada et al. (2021-10-04), Retinal VEGF-A Overexpression Is Not Sufficient to Induce Lymphangiogenesis Regardless of VEGF-C Upregulation and Lyve1+ Macrophage Infiltration Investigative Ophthalmology Visual Science, 2021-10-04;62(13):17.
PMID: 34673901
Species: Human
Sample Types: Serum

K Shuto et al. (2019-01-25), Hepatic blood flow by perfusion computed tomography as an imaging biomarker for patients with gastric cancer Oncol Lett, 2019-017(3):3267-3276.
PMID: 30867759
Species: Human
Sample Types: Plasma

Kumar N et al. (2016-10-04), Tuberculosis-diabetes co-morbidity is characterized by heightened systemic levels of circulating angiogenic factors. J Infect, 2016-174(1):10-21.
PMID: 27717783
Species: Human
Sample Types: Plasma

Kumar N et al. (2016-01-04), Circulating Angiogenic Factors as Biomarkers of Disease Severity and Bacterial Burden in Pulmonary Tuberculosis. PLoS ONE, 2016-011(1):e0146318.
PMID: 26727122
Species: Human
Sample Types: Serum

Conroy A et al. (2014-01-20), Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-014(0):35.
PMID: 24444080
Species: Human
Sample Types: Plasma

Munaut C et al. (2012-03-12), Differential expression of Vegfr-2 and its soluble form in preeclampsia. PLoS ONE, 2012-07(3):e33475.
PMID: 22428059
Species: Human
Sample Types: Cell Culture Supernates

Kishuku M et al. (2009-12-15), Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. J. Immunol., 2009-1183(12):8176-85.
PMID: 20007583

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Product Documents for Human sVEGFR2/KDR DuoSet ELISA

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